Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (30): 4775-4779.doi: 10.3969/j.issn.2095-4344.1403

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Preparation of nano-demineralized bone matrix by modified low temperature mechanical trituration 

Wang Zhiwei, Yuan Bo, Chen Xiongsheng, Zhou Shengyuan, Jia Lianshun
  

  1. Spine Center, Department of Orthopedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China
  • Received:2019-04-21 Online:2019-10-28 Published:2019-10-28
  • Contact: Chen Xiongsheng, MD, Doctoral supervisor, Chief physician, Professor, Spine Center, Department of Orthopedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China
  • About author:Wang Zhiwei, MD, Spine Center, Department of Orthopedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China
  • Supported by:

    The Major Project of the Science and Technology Commission of Shanghai Municipality, No. 15JC1491003 (to CXS)

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Abstract:

BACKGROUND: Previous studies have confirmed that nano-demineralized bone matrix is an alternative to an autograft with non-toxicity, good tissue compatibility, high bioavailability, mild inflammatory reaction, strong osteogenic ability. But its preparation takes long time. Intermittent sampling is required for particle size analysis.
OBJECTIVE: To prepare nano-demineralized bone matrix by low temperature mechanical trituration and analyze its structure characteristics and preparation efficacy.
METHODS: Allogeneic decalcified bone matrix was prepared by the modified Urist method. The CryoMill automatic freezing grinder was used to initially grind the decalcified bone matrix to the micron-sized particles. Then the E-Max high-energy water-cooled ball mill was used to grind the micro-sized particles into nano-sized particles. Finally, the grinding effect and material appearance were observed by scanning electron microscopy.
RESULTS AND CONCLUSION: After two-step grinding, the prepared decalcified bone matrix had nanostructure composed by irregular 20-50 nm-sized nanoparticles through agglomeration. The surface of nano-particles was densely covered with nano-scale grooves. A large number of interconnected micro-pores formed between nanofiber structures, the microstructure of which conformed to the category of nanobiomaterials. Compared with the Micro superfine mill, the new trituration process only took 25 minutes, which increases grinding efficacy. These results suggest that two steps of low temperature mechanical trituration for preparation of nano-demineralized bone matrix produces more uniform particle size distribution.

Key words: nanomaterials, nano-demineralized bone matrix, demineralized bone matrix, nanocrystallization, low temperature mechanical trituration, micro-characteristic, trituration

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