Association of interleukin-6 polymorphism with serum level of interleukin-6 in endurance athletes

doi:10.3969/j.issn.2095-4344.0853

Abstract

Abstract:

BACKGROUND: Aerobic capacity has a high heritability, and with the development of molecular biotechnology, molecular genetic marker screening is helpful for gene diagnosis and predicting aerobic capacity.
OBJECTIVE: To observe the distribution characteristics of single nucleotide polymorphism (SNP) of interleukin-6 gene in endurance athletes, explore whether it can be used as a molecular genetic marker, and whether there is any association between interleukin-6 SNP and serum level of interleukin-6.
METHODS: Two groups of subjects were selected. In the endurance group, 78 athletes of Han nationality and from northern plains who were engaged in endurance project (middle distance race, marathon race, or heel-and-toe walking race) for at least 2 years, and at least second-class sports grade were included. In the control group, 142 subjects who were Beijing city middle school students (nonobese or low body mass, 12-15 years old), and whose parents were of Han nationality from northern plains were included. Three SNP loci of interleukin-6 gene rs1800796 (-572C/G), rs2066992 (1430T/G) and rs13306436 (4477G/A) were typed by PCR-RFLP, and the serum level of interleukin-6 was tested by ELISA.
RESULTS AND CONCLUSION: There was gender difference in -572C/G genotype distribution in the endurance group (P < 0.05), and the genotype distribution of the female endurance group was higher than that of the female control group (P=0.095). In the female endurance group CC genotype accounted for 61.1%, and CG+GG genotype accounted for 38.9%. In the female control group, CC genotype accounted for 43.0, and CG+GG genotype accounted for 57.0. The serum level of interleukin-6 of female athletes with -572C/G locus CC genotype in the female endurance group was lower than that in the female control group (P < 0.05). In addition, the serum level of interleukin-6 in athletes with -572C/G locus CC genotype in the endurance group was lower than that in the control group (P=0.070). The rs1800796 of interleukin-6 gene is a molecular marker of female endurance athletes. The serum level of interleukin-6 in female athletes with CC genotype at rs1800796 is lower than that in female controls. Rs1800796, rs2066992 and rs13306436 of interleukin-6 are not genetic markers for male athletes. Further study should focus on expanding the sample size.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Interleukin 6, Polymorphism, Single Nucleotide, Genes, Tissue Engineering

CLC Number:

R318

Chen Jun-fei1, 2, Yan Yi1, Guo Jian-jun3, Sun Ming-xiao4, Tang Qiang2, Xie Min-hao5. Association of interleukin-6 polymorphism with serum level of interleukin-6 in endurance athletes[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(32): 5215-5221.

Figures/Tables 4

2.1 参与者数量分析试验纳入受试者220名，测试过程无脱落，全部进入结果分析。 2.2 耐力组和对照组白细胞介素6基因-572C/G位点多态性分布特征白细胞介素6基因-572C/G位点基因解析结果如图1所示。耐力组、男耐力组、女子耐力组、对照组、男对照组、女子对照组每组P值都大于0.05，各组均符合Hardy-Weinberg平衡检验。基因型和等位基因分布特征如表2所示。基因型分布：对照组中基因型的分布没有性别差异，耐力组中性别差异显著。其中，男对照组与女子对照组：χ2=1.106，df=2，P=0.575；男耐力组与女子耐力组： χ2=6.019，df=1，P=0.014(CG+GG)。基因型的分布在耐力组与对照组间没有显著性差异。其中，男耐力组与男对照组：χ2=1.628，df=2，P=0.443；女子耐力组与女子对照组：χ2=4.716，df=2，P=0.095。等位基因分布：耐力组和对照组中等位基因的分布没有性别差异。其中，男对照组与女子对照组：χ2=0.229，df=1，P=0.632；男耐力组与女子耐力组：χ2=3.353，df=1，P=0.081。等位基因的分布在耐力组与对照组没有显著性差异。其中，男耐力组与男对照组：χ2=0.162，df=1，P=0.687；女子耐力组与女子对照组：χ2=1.426，df=1，P=0.232。 2.3 耐力组和对照组白细胞介素6基因1430T/G位点多态性分布特征白细胞介素6基因1430T/G位点基因解析结果如图2所示。耐力组、男耐力组、女子耐力组、对照组、男对照组、女子对照组每组P值都大于0.05，各组均符合Hardy-Weinberg平衡检验。基因型和等位基因分布特征如表3所示。基因型分布：对照组和耐力组中基因型的分布没有性别差异。其中，男对照组与女子对照组：χ2=1.081，df=2，P=0.583；男耐力组与女子耐力组：χ2=3.893，df=1，P=0.048(TG+GG)。基因型的分布在耐力组与对照组间也没有显著性差异。其中，男耐力组与男对照组：χ2=1.680，df=1，P=0.195；女子耐力组与女子对照组：χ2=0.240，df=1，P=0.624。等位基因分布：耐力组和对照组中等位基因的分布没有性别差异。其中，男对照组与女子对照组：χ2=0.748，df=1，P=0.387；男耐力组与女子耐力组：χ2=1.857，df=1，P=0.173。等位基因的分布在耐力组与对照组也没有显著性差异。其中，男耐力组与男对照组：χ2=0.402，df=1，P=0.526；女子耐力组与女子对照组：χ2=0.047，df=1，P=0.828。 2.4 耐力组和对照组白细胞介素6基因4477G/A位点多态性的分布特征白细胞介素6基因4477G/A位点基因解析结果如图3所示。耐力组、男耐力组、女子耐力组、对照组、男对照组、女子对照组每组P值都大于0.05，各组均符合Hardy-Weinberg平衡检验。基因型和等位基因分布特征如表4所示。"

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