Exosomes from human umbilical cord mesenchymal stem cells at passage 2 and 5: a comparative study on microRNA profiles

doi:10.3969/j.issn.2095-4344.0525

Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (17): 2686-2691.doi: 10.3969/j.issn.2095-4344.0525

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Exosomes from human umbilical cord mesenchymal stem cells at passage 2 and 5: a comparative study on microRNA profiles

Lin Qing-keng^{1, 2}, Yin De-hong², Liu Ju-fen², Zhang Xue-juan^{1, 2}, Bai Ying-ying^{1, 2}, Song Yi-jia^{1, 2}, Pan Xing-hua², Liu-Gao Mi-yang²

¹Kunming General Hospital Clinical College of Kunming Medical University, Kunming 650032, Yunnan Province, China; ²Cell Biological Therapy Center of Kunming General Hospital of PLA, Cell Biological Medicine integrated Engineering Laboratory of State and Region of Yunnan Province, the Stem Cell Therapy Key Laboratory of Yunnan Province, Kunming 650032, Yunnan Province, China

Revised:2018-04-17 Online:2018-06-18 Published:2018-06-18
Contact: Liu-Gao Mi-yang, Master, Cell Biological Therapy Center of Kunming General Hospital of PLA, Cell Biological Medicine integrated Engineering Laboratory of State and Region of Yunnan Province, the Stem Cell Therapy Key Laboratory of Yunnan Province, Kunming 650032, Yunnan Province, China
About author:Lin Qing-keng, Master candidate, Kunming General Hospital Clinical College of Kunming Medical University, Kunming 650032, Yunnan Province, China; Cell Biological Therapy Center of Kunming General Hospital of PLA, Cell Biological Medicine integrated Engineering Laboratory of State and Region of Yunnan Province, the Stem Cell Therapy Key Laboratory of Yunnan Province, Kunming 650032, Yunnan Province, China. Yin De-hong, Attending physician, Cell Biological Therapy Center of Kunming General Hospital of PLA, Cell Biological Medicine integrated Engineering Laboratory of State and Region of Yunnan Province, the Stem Cell Therapy Key Laboratory of Yunnan Province, Kunming 650032, Yunnan Province, China. Lin Qing-keng and Yin De-hong contributed equally to this work.
Supported by:
the Animal Special Fund of PLA, No. SYDW(2016)004; the Key Laboratory Project of Yunnan Province, No. 2015DG034

Abstract

Abstract:

BACKGROUND: Exosomes have the function of some mesenchymal stem cells. Understanding the substance composition that plays a representative role in mesenchymal stem cell exosomes will provide clues for further exploration of synthetic exosome analogues.
OBJECTIVE: To investigate the difference of microRNA expression profiles in exosomes derived from passage 2 and 5 human umbilical cord mesenchymal stem cells (hUC-MSCs).
METHODS: Exosomes in the supernatant of passage 2 and 5 hUC-MSCs were extracted by ultra-high speed centrifugation. The established library was sequenced by using high-throughput sequencing technology. Then we analyzed the sequence results so as to understand the microRNA expression between different groups, and finally did a cluster analysis.
RESULTS AND CONCLUSION: 427 657 kinds of microRNAs were detected in the exosomes from passage 2 hUC-MSCs, accounting for 68.93% of the total microRNAs detected; and 119283 microRNAs were detected in the exosomes from passage 5 hUC-MSCs, accounting for 19.22% of the total microRNAs detected. There were 73 526 microRNAs shared between the exosomes from passage 2 and passage 5 hUC-MSCs, accounting for 11.85% of the total microRNAs detected. Bioinformatics analysis (cluster analysis) results showed that these miRNAs were likely to be involved in 161 biological processes, including cell repair, immune and anti-aging. The microRNAs in exosomes from passage 2 to passage 5 hUC-MSCs were largely different. Partial miRNAs exhibited significantly reduced copy numbers. The top five microRNAs with a higher amount, including has-miR-146a-5p, has-miR-191-5p, has-miR-493-3p, has-miR-423-5p, and has-miR-134-5p, have the potential to be the component of synthetic exosome analogues.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Umbilical Cord, Mesenchymal Stem Cells, Exosomes, MicroRNAs, High-Throughput Screening Assays, Tissue Engineering

CLC Number:

R394.2

Lin Qing-keng, Yin De-hong, Liu Ju-fen, Zhang Xue-juan, Bai Ying-ying, Song Yi-jia, Pan Xing-hua, Liu-Gao Mi-yang. Exosomes from human umbilical cord mesenchymal stem cells at passage 2 and 5: a comparative study on microRNA profiles[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(17): 2686-2691.

Figures/Tables 2

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Exosomes from human umbilical cord mesenchymal stem cells at passage 2 and 5: a comparative study on microRNA profiles

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