Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (24): 4526-4529.doi: 10.3969/j.issn.1673-8225.2012.24.032

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Characterization and analysis of matrix metalloproteinases 8 and 20 in the human crown and root dentin

Zhu Zi-yuan, Zhou Tian, Zhang Bao-wei   

  1. Department of Prosthodontics, Shanghai Key Laboratory of Stomatology, College of Stomatology, the Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China
  • Received:2011-12-01 Revised:2012-01-13 Online:2012-06-10 Published:2013-11-05
  • Contact: Zhang Bao-wei, Chief physician, Department of Prosthodontics, Shanghai Key Laboratory of Stomatology, College of Stomatology, the Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China baoweizhang18@msn.com
  • About author:Zhu Zi-yuan☆, Doctor, Attending physician, Department of Prosthodontics, Shanghai Key Laboratory of Stomatology, College of Stomatology, the Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China yuan1588@hotmail.com

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Abstract:

BACKGROUND: In recent years, matrix metalloproteinases (MMP)-mediated collagen degradation in dentin has been widely studied. Recent studies have shown that MMPs play an important role in the progress of periodontal disease, dentin caries, pulpitis and failure in dentin adhesive interface.
OBJECTIVE: To determine the contents and distribution features of MMP-8 and -20 in the human crown and root dentin.
METHODS: Dentin powder was extracted by guanidine hydrochloride, then subjected to ethylene diamine tetraacetic acid demineralization in four cycles, and finally extracted by guanidine hydrochloride again. Extracts were analyzed by Western blot and enzyme linked immunosorbent assay (ELISA) for MMP-8 and -20 detection.
RESULTS AND CONCLUSION: MMP-8 and MMP-20 in the extracted materials were confirmed by western blot and ELISA. MMP-8 and -20 were mostly extracted from the mineralized compartment of the dentin (E1). These two MMPs presented overall lower levels in G1 and the lowest levels in G2. Both Western blot analysis and ELISA detected that MMP-8 and -20 equally distributed in the crown and root dentin. MMP-8 and MMP-20 proteins have been found in the extracted materials from the human crown and root dentin. They are mostly presented in mineralized dentin, both in the crown and root dentin.

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