Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 1036-1040.doi: 10.3969/j.issn.1673-8225.2012.06.020

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Therapeutic effect of erythropoietin gene modified umbilical cord derived mesenchymal stem cells transplantation on the morphology of brain cells and the recovery of neurological function in traumatic brain injury rats

Zhong Zhi-jian, Lü Jia-xi   

  1. Department of Neurosurgery, Guilin Second People's Hospital, Guilin  541001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-07-05 Revised:2011-09-30 Online:2012-02-05 Published:2012-02-05
  • About author:Zhong Zhi-jian, Attending physician, Department of Neurosurgery, Guilin Second People's Hospital, Guilin 541001, Guangxi Zhuang Autonomous Region, China zzj-9898@163.com

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Abstract:

BACKGROUND: Many studies have shown that erythropoietin (EPO) has a neuroprotective effect.
OBJECTIVE: To investigate the therapeutic effect of EPO modified human umbilical cord derived mesenchymal stem cells (UC-MSCs) intracerebral transplantation on traumatic brain injury of rats.
METHODS: The expression of exogenous EPO in UC-MSCs was examined by Western blot. 90 healthy Wistar rats were used to establish the heavy-duty hydraulic model of traumatic brain injury. The models were randomly divided into control group, UC-MSCs transplantation group and EPO+UC-MSCs transplantation group.
RESULTS AND CONCLUSION: Western blot results showed that the EPO gene modified UC-MSCs could express the EPO in vitro. Compared with control group, the neurological severity scores (mNSS) in UC-MSCs transplantation group and EPO+UC-MSCs transplantation group was decreased (P < 0.05) and lower in EPO+UC-MSCs transplantation group (P < 0.01). The average time of escape latency was gradually decreased in each group. However, from the 3rd to the 5th day, time in EPO+UC-MSCs transplantation group was shorter than that in control group (P < 0.05) and then in UC-MSCs group (P < 0.01). In addition, the frequency of platform passing and the percentage of swimming distance in target quadrant and total distance was highest in EP0+UC-MSCs transplantation group (P < 0.05). Morphological observation showed that the scar and malacia of brain tissue in EPO+UC-MSCs transplantation group was smaller than that in UC-MSCs transplantation group and then in control group, but the sequence of the CM-Dil positive cells number in three groups was contrast. Transplantation of EPO gene-modified UC-MSCs can significantly improve the neurological function in the rats with TBI.  

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