Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 1032-1035.doi: 10.3969/j.issn.1673-8225.2012.06.019

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Intravenous administration of human umbilical cord blood stem cells reduces nerve cell apoptosis of cerebral ischemia rats

Song Hong1, Fu Xia2, Dong Lei-lei1   

  1. 1Department of Neurology, Shenzhou Hospital of Shenyang Medical College, Shenyang  110002, Liaoning Province, China; 2Liaoning University Hospital, Shenyang  110036, Liaoning Province, China
  • Received:2011-07-21 Revised:2011-09-30 Online:2012-02-05 Published:2012-02-05
  • Contact: Fu Xia, Master, Chief physician, Liaoning University Hospital, Shenyang 110036, Liaoning Province, China fu-xia@sohu.com
  • About author:Song Hong★, Master, Attending physician, Department of Neurology, Shenzhou Hospital of Shenyang Medical College, Shenyang 110002, Liaoning Province, China songhong_zyn@126.com

Abstract:

BACKGROUND: After transplantation, the umbilical cord blood stem cells can migrate to the brain damage zone, survive and differentiate into nerve cells, promote recovery of damaged neurological function.
OBJECTIVE: To approach the curative effect of intravenous administration of human umbilical cord blood stem cells (hUCBSCs) on cerebral ischemia in rats, and its influence on nerve cells apoptosis.
METHODS: The middle cerebral artery occlusion (MCAO) model was established by using Zea-Longa’s method in rats. 48 rats were randomly divided into two groups: control group (n=24) and treatment group (n=24). Control group and treatment group were received intravenous administration of PBS and hUCBSCs, respectively, at 1 day after operation.
RESULTS AND CONCLUSION: There were no significant difference between the neurological scores of two groups at 3 and 7 days after operation (P >0.05); at the 14th and 21st days after operation, the neurological score was significantly improved in the treatment group compared with the control group (P < 0.05). Many BrdU-positive cells were found in the cerebral section in treatment group, while no BrdU-positive cells were found in control group. The number of apoptotic cells in treatment group was significantly less than that in the control group (P < 0.05). Intravenously administered hUCBSCs could migrate to the ischemia brain tissue, inhibit nerve cell apoptosis and improve the neurological function significantly.

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