Abstract:

BACKGROUND: The bone marrow stem cells can be mobilized and migrate to the damage tissue after the normal tissue defect and can proliferate and differentiate and repair the damage tissue under local microenvironment.
OBJECTIVE: To establish the acute ischemic myocardial infarction model of swines mobilized by colony-stimulating factor (CSF) and to study the effect of bone marrow mobilization on the cardiac function and vascular regeneration of acute myocardial infarction model.
METHODS: Fifteen healthy Taihu Meishan swines were used to establish the ischemic myocardial infarction model mobilization in the distal end of the left anterior descending branch (LAD) with a gelatin sponge through cardiac catheter, and explore its mechanism initially. The swines were randomly divided into three groups: the control group, injected with DMEM through coronary artery at the 4th week after modeling; the immediate mobilization group, injected with granulocyte colony-stimulating factor (G-CSF) at the 3rd hour after modeling and last for 5 days; 1 week mobilization group, injected with G-CSF at the 1st week after modeling and last for 5 days.
RESULTS AND CONCLUSION: Compared with control group, the functional parameter, such as ejection fraction, left ventricular internal dimension diastole and left ventricular internal dimension systole in immediate mobilization group and 1 week mobilization group were improved significantly. And after bone marrow mobilization, the serum levels of vascular endothelial growth factor (VEGF) in the two groups were significantly elevated, as well as the vascular density. There was no obvious difference in control group. Bone marrow stem cells were mobilized by G-CSF and migrated to the site of myocardial infarction, and differentiated into initial vascular structure, and take part in the vascular formation in ischemic myocardium.