Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 985-988.doi: 10.3969/j.issn.1673-8225.2012.06.008

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N-acetylcysteine protects bone marrow stromal cells against the toxicity of 6-hydroxydopamine★

Zhang Qi-lin, Luo Wei-feng, Wang Heng-hui, Ye Yan, Zhu Ting-ge, Liu Chun-feng   

  1. Department of Neurology, Second Affiliated Hospital of Soochow University, Suzhou  215004, Jiangsu Province, China
  • Received:2011-07-23 Revised:2011-08-05 Online:2012-02-05 Published:2012-02-05
  • Contact: Luo Wei-feng, Chief physician, Master’s supervisor, Department of Neurology, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China lwfwxx@126.com
  • About author:Zhang Qi-lin★, Master, Physician, Department of Neurology, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China zhangqilin1984@yahoo.com.cn

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Abstract:

BACKGROUND: 6-hydroxydopamine, as an endogenous toxic factor in the pathogenesis of Parkinson's disease, participates in oxidative stress. N-acetylcysteine resists oxidation and removes free radicals effectively.
OBJECTIVE: To investigate the toxicity of 6-hydroxydopamine in bone marrow stromal cells and the antagonistic effect of N-acetylcysteine on it.
METHODS: Bone marrow stromal cells of Sprague-Dawley rats were cultured in vitro. Bone marrow stromal cells of passage 3 were treated with 6-hydroxydopamine with the final concentrations of 0, 0.05, 0.1 g/L and N-acetylcysteine with the final concentrations of 0, 0.075, 0.3, 1.2, 4.8 g/L, respectively.
RESULTS AND CONCLUSION: MTT assay showed that 6-hydroxydopamine (0.05 and 0.1 g/L) significantly decreased the viability of bone marrow stromal cells. This toxic effect of 6-hydroxydopamine was significantly inhibited by 0.3 g/L N-acetylcysteine. It suggests that antioxidant N-acetylcysteine may affect the toxic action of 6-hydroxydopamine.

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