Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 981-984.doi: 10.3969/j.issn.1673-8225.2012.06.007

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Effects of alcohol on the expression of neuropeptide in human bone marrow mesenchymal stem cells  

Wang Yi-sheng1, Zhu Shao-yang1, Li Yue-bai2, Zhao Guo-qiang3, Zhang Zhan-feng1   

  1. 1Departmemt of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Open Laboratory of Key Subjects of Clinical Medicine of Key Subjects, Zhengzhou  450052, Henan Province, China; 2Department of Biochemistry; 3Department of Microbiology & Immunology, Basic Medical College of Zhengzhou University, Zhengzhou  450001, Henan Province, China
  • Received:2011-11-28 Revised:2011-12-20 Online:2012-02-05 Published:2012-02-05
  • About author:Wang Yi-sheng★, Master, Professor, Chief physician, Doctoral supervisor, Departmemt of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Open Laboratory of Key Subjects of Clinical Medicine of Henan Province, Zhengzhou 450052, Henan Province, China wangyisheng@zzu.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No.81171776*

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Abstract:

BACKGROUND: Studies have found that the nervous system can effect the differentiation of bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To investigate the effect of alcohol on the expressions of calcitontin gene-related peptide receptor (CGRPR) mRNA, substance P receptor (SPR) mRNA, peroxisom proliferator-activeted receptor-γ (PPARγ) mRNA and Runx2 mRNA in human BMSCs. 
METHODS: The second generation of BMSCs were divided into two groups at random. The cells in experimental group were treated with 0. 09 mol/L alcohol, and the control group was not treated.
RESULTS AND CONCLUSION: The expressions of FGF mRNA, CGRPR mRNA, SPR mRNA and Runx2 mRNA in the experimental group were significantly lower those that in the control group (P < 0.01), but the expression of PPARγ mRNA was increased compared with control group (P < 0.01). Alcohol could change the expressions of neuropeptide and depress the differentiation to osteoblasts.    

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