Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 977-980.doi: 10.3969/j.issn.1673-8225.2012.06.006

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Effects of α-zeranol on expression of osteoprotegerin in bone marrow mesenchymal stem cells-derived osteoblasts  

Liu Wei-hua1, Duan Fei1, Li Shao-chun1, Sun Xiao-fang1, Hao Hua-chao1, He Jing1, Xue Juan1, Deng Xin1, Luo Lei-lei1, Guo Wen-long2   

  1. 1School of Basic Medical Science, Hebei University, Baoding  071000, Hebei Province, China; 2Department of Emergency, Laishui Hospital, Laishui  074100, Hebei Province, China
  • Received:2011-12-01 Revised:2012-01-04 Online:2012-02-05 Published:2012-02-05
  • Contact: Duan Fei, Doctor, Professor, Doctoral supervisor, School of Basic Medical Science, Hebei University, Baoding 071000, Hebei Province, China feiduan9999@sina.com
  • About author:Liu Wei-hua, Teaching assistant, School of Basic Medical Science, Hebei University, Baoding 071000, Hebei Province, China doctorliu09@163.com
  • Supported by:

    Population and Family Planning Commission Project of Hebei Province, No. 2010-A03*; Pre-research Fund Project of Hebei University School of Basic Medical Science, No.2007JY04*; Special Fund for Subject Construction Project of Hebei University School of Basic Medical Science No.2012A2002*

Abstract:

BACKGROUND: Studies showed that α-zeranol (α-ZAL) has significant prevention and treatment effect on postmenopausal osteoporosis.
OBJECTIVE: To observe the effects of α-ZAL on the secretion of osteoprotegerin (OPG) and expression of OPG mRNA in osteoblasts of the rats.
METHODS: Estrogen and α-ZAL (10-5-10-10 mol/L) were added into in vitro cultured osteoblasts respectively for intervention.
RESULTS AND CONCLUSION: The results of ELISA and RT-PCR showed that after application of different concentrations of α-ZAL (10-5-10-10 mol/L), the secretion of OPG and expression of OPG mRNA in the estrogen group were higher than those in the control group (P < 0.05). The results confirmed that in the bone marrow mesenchymal stem cells-derived osteoblast, adding α-ZAL can promote osteoblast OPG secretion and mRNA expression.
 

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