Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 973-976.doi: 10.3969/j.issn.1673-8225.2012.06.005

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Effect of different sources of bone mesenchymal stem cells on CD34+ cell proliferation

Wang Rong1, Liu Rui-feng2, Zhang Yong-cui2, Pan Zhi-hui2, Li Xin2, Liu Xiao-min3, Yin Guo-hua2, Zhang Jing4, Zhang Kai-ming2   

  1. 1Second Clinical Medical College of Shanxi Medical University, Taiyuan  030001, Shanxi Province, China; 2Department of Dermatology, Taiyuan Central Hospital, Taiyuan  030009, Shanxi Province, China; 3Department of Inter Medicine, Hospital of Zhongbei University, Taiyuan 030051, Shanxi Province, China; 4Department of Cardiology, Taiyuan Central Hospital, Taiyuan  030009, Shanxi Province, China
  • Received:2011-07-16 Revised:2011-09-27 Online:2012-02-05 Published:2012-02-05
  • Contact: Zhang Jing, Chief physician, Department of Cardiology, Taiyuan Central Hospital, Taiyuan 030009, Shanxi Province, China zhangjing@sina.com
  • About author:Wang Rong★, Studying for master’s degree, Second Clinical Medical College of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China wrsuny@126.com
  • Supported by:

    the National Natural Science Foundation of China, No.30972657*; Shanxi Natural Science Foundation, No.2008011082-2*

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Abstract:

BACKGROUND: There are some significant differences of biological characteristics, immunological reaction and antigen presentation between bone mesenchymal stem cells (BMSCs) from psoriasis vulgaris (PV) patients and normal health. But there are no more differences between PV patients and aborted fetus.
OBJECTIVE: To study the effect of different sources of BMSCs on CD34+ cell proliferation.
METHODS: Mononuclear cells were separated from bone marrow in PV patients and aborted fetus by density gradient centrifugation method, then the cells were cultivated and generated. The cultured supernatant was collected until 72 hours later by spreading to the second generation. The purity of CD34+ cells and BMSCs was detected by flow cytometry. Cells growth was observed under inverted microscope. CD34+ cells from normal human bone marrow were selected by magnetic cell sorting method, and then the cells were cultured in the BMSCs culture supernatant for 24 hours. MTT was used to detect the proliferative activity of CD34+ cells.
RESULTS AND CONCLUSION: There was no statistic difference on the morphology of CD34+ cells after cultured in the BMSCs culture supernatant derived from PV patients and aborted fetus for 24 hours. There is no significantly effect of BMSCs culture supernatant derived from PV patients and aborted fetus on the proliferation of CD34+ cells (P > 0.05).
 

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