Abstract:

BACKGROUND: Ischemia/reperfusion injury is the inevitable pathophysiological process in clinical organ transplantation. Cold ischemia/reperfusion injury has more targeted in organ transplantation process.
OBJECTIVE: To investigate the potential role and intervention of high mobility group box-1 (HMGB1) protein in rats with renal cold ischemia/reperfusion injury．
METHODS: SD rats were randomly divided into three groups: sham-operated group (n=20), cold ischemia/reperfusion injury group (n=20), and treatment group (n=20). Rats in the cold ischemia/reperfusion injury group and treatment groups were respectively administered Ringer’s solution and EP via the penile vein before preparation of cold ischemia/reperfusion injury models. The sham-operated group rats were administered Ringer’s solution after opening the abdominal cavity, and 45 minutes later, the abdominal cavity was closed.
RESULTS AND CONCLUSION: Compared with sham-operated group, creatinine, HMGB1 protein, tumor necrosis factor-α and nuclear factor-κB levels were significantly higher in the cold ischemia-reperfusion injury group and treatment group (P < 0.01). The above indices were significantly higher in the cold ischemia-reperfusion injury group than in the treatment group (P < 0.01). These findings suggest that the HMGBl plays an important role in the pathological processes of renal cold ischemia reperfusion injury, and EP can reduce renal cold ischemia-reperfusion injury.