Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (44): 8205-8208.doi: 10.3969/j.issn.1673-8225.2011.44.010

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Primary culture of bovine chondrocyte-like nucleus pulposus cells and cell histocompatibilty in xenotransplantation

Hou Xiao-fei, Li Hao-peng, Yang Bao-hui   

  1. Second Department of Orthopedics, Second Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an  710004, Shaanxi Province, China
  • Received:2011-06-05 Revised:2011-09-06 Online:2011-10-29 Published:2011-10-29
  • Contact: Li Hao-peng, Chief physician, Master’s supervisor, Second Department of Orthopedics, Second Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China lhp-3993@163.com
  • About author:Hou Xiao-fei★, Studying for master’s degree, Second Department of Orthopedics, Second Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China houxiaofie3718@163.com

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Abstract:

BACKGROUND: Autologous or allogenic cell transplantation for regeneration of intervertebral discs has become a hot spot in clinical study.
OBJECTIVE: To investigate primary culture method of bovine chondrocyte-like nucleus pulposus cells and observe cell histocompatibility after transplantation into rabbit intervertebral discs.
METHODS: Fresh bovine caudal nuclesus pulposus was collected. Bovine chondrocyte-like nucleus pulposus cells were isolated, sequentially digested by pronase E and collagenase Ⅱ and cultured in DMEM/F12 medium and 10 %fetal bovine serum. The expanded cells were injected into rabbit intervertebral discs through the use of microinjector via the posterolateral approach. At 2, 4, 6 and 8 weeks after transplantation, rabbit intervertebral discs in which bovine caudal nuclesus pulposus cells were transplanted. CD5 or CD4 cell surface antigen expression and B or T lymphocyte infiltration were detected using immunohistochemistry.
RESULTS AND CONCLUSION: Bovine chondrocyte-like cells were isolated successfully. Their number was expanded in vitro. At 2, 4, 6 and 8 weeks after transplantation, no anti-rabbit CD4 and CD5 immunoactive expression or C or T lymphocyte infiltration was observed in any treated intervertebral discs. At 8 weeks after transplantation, no immunological rejection was observed, as shown by immunohistochemistry. These findings suggest that primary cultured bovine chondrocyte-like nuclesus pulposus shows good histocompatibility.

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