Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7505-7510.doi: 10.3969/j.issn.1673-8225.2011.40.022

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Effects of ganglioside combined with neural stem cells transplantation on the neurologic function of rats with traumatic brain injury

Li Hong-xing1, Yuan Guo-fu1, Jin Yao-dong2, Wang Jing3, Zhao Zong-mao4   

  1. 1Department of Neurosurgery, 2Department of Emergency, 3Department of Phanrmacy, People's Hospital, Xushui  072550, Hebei Province, China; 4Department of Neurosurgery, Second Affiliated Hospital of Hebei Medical University Shijiazhuang  050000, Hebei Province, China
  • Received:2010-10-28 Revised:2010-12-16 Online:2011-10-01 Published:2011-10-01
  • Contact: Yuan Guo-fu, Master, Attending physician, Department of Neurosurgery, Xushui People's Hospital, Xushui 072550, Hebei Province, China sheibobobo@163.com
  • About author:Li Hong-xing, Attending physician, Department of Neurosurgery, Xushui People's Hospital, Xushui 072550, Hebei Province, China Lhxwj123@sina.com
  • Supported by:

    Hebei Provincial Medical Research Project, No.20110598*

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Abstract:

BACKGROUND: Ganglioside (GM1), through activation of neurotrophic factors, inhibits the damage of toxic products to neurons and reduces nerve cell death induced by excitatory amino acids to play a role in nerve cell repair.
OBJECTIVE: To investigate the effect of neural stem cells (NSCs) transplantation and GM1 on traumatic brain injury (TBI).
METHODS: Healthy Wistar rats were divided randomly into three groups: TBI group; NSCs transplantation group; NSCs transplantation+ GM1 group. AQP4 mRNA and AQP4 expressions were determined by RT-PCR and Western Blot at 4 day after TBI. The neurological defect scores were determined 24 hours, 3 days and 1, 2, 3, 4 weeks after TBI. The Morris water maze test was tested on days 21-28 after TBI. The immunohistochemistry and pathology changes were checked after 4 weeks.
RESULTS AND CONCLUSION: The expressions of AQP4 mRNA and AQP4 were highest in the TBI group, higher in the NSCs transplantation group and lowest in the NSCs transplantation+ GM1 group (P < 0.05). At 1, 2, 3 and 4 weeks after TBI, the neurological defect scores were lower in the NSCs transplantation group than those in the TBI group (P < 0.05), and significantly lower in the NSCs transplantation+GM1 group than those in the TBI group (P < 0.01); The average time of escape latency was gradually decreased in each group. However, from the 3rd to 5th day, it was much shorter in the NSCs transplantation+GM1 group than in the TBI group (P < 0.01) and shorter in the NSCs transplantation+GM1 group than in NSCs transplantation group (P < 0.05). In addition, the frequency platform passing in the NSCs transplantation+GM1 group and the percentage of swimming distance traveled in the previous target quadrant was significantly greater than those of the TBI group and NSCs transplantation group. The number of neurons in the NSCs transplantation+GM1 group was higher than that in the NSCs transplantation group and TBI group (P < 0.05). GM1 combined with neural stem cells transplantation can significantly improve the neurological function in the rats with TBI. 

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