Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7429-7432.doi: 10.3969/j.issn.1673-8225.2011.40.005

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Diethylstilbestrol can induce bone marrow stromal cells to differentiate into osteoblasts

Zhang Chang-hai1, Zhang Xian-cheng1, Hu Meng1, Xue Zheng-min1, Zhou Xiao-peng1, Li Cui-yun2   

  1. 1Department of Spinal Surgery, Second People’s Hospital of Jining City, Jining  272000, Shandong Province, China
    2Department of Radiation Oncology, Jining Tumor Hospital, Jining  272000, Shandong Province, China
  • Received:2011-01-25 Revised:2011-03-23 Online:2011-10-01 Published:2011-10-01
  • Contact: Hu Meng, Master, Physician, Department of Spinal Surgery, Second People’s Hospital of Jining City, Jining 272000, Shandong Province, China
  • About author:Zhang Chang-hai, Associate chief physician, Department of Spinal Surgery, Second People’s Hospital of Jining City, Jining 272000, Shandong Province, China Humeng810423@163.com

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Abstract:

BACKGROUND: There are fewer reports about estrogen effects on bone marrow stromal cells (BMSCs).
OBJECTIVE: To study the effect of diethylstilbestrol on the osteogenic differentiation of rabbit BMSCs.
METHODS: Rabbit BMSCs cultured in vitro were intervened with 0, 10-7, 10-6, 10-5 mol/L diethylstilbestrol, and BMSCs cultured with dexamethasone 10-8 mol/L, β-sodium glycerophosphate 10 mmol/L, and vitamin C 50 mg/L were used as positive controls.
RESULTS AND CONCLUSION: 10-6 mol/L diethylstilbestrol significantly improved the proliferative ability of BMSCs at 24, 48, and 72 hours after intervention (P < 0.01). 10-5 mol/L diethylstilbestrol significantly inhibited the proliferation of BMSCs at 48 hours after intervention as well as 10-7 mol/L diethylstilbestrol at 72 hours (P < 0.01). Mineralized nodular structures formed at 25 days after intervention with 10-7 mol/L diethylstilbestrol. Alkaline phosphatase activities were remarkably increased at 14 and 21 days after intervention with 10-7, 10-6 mol/L diethylstilbestrol. It has been proved that diethylstilbestrol has an enhancing effect on the osteogenic differentiation of rabbit BMSCs.

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