Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have multiple differentiation potential in vitro, but the in vitro differentiation of BMSCs to the lung tissue is still controversial.
OBJECTIVE: To verify the ability of mouse BMSCs differentiating to endothelial cells and epithelial cells in vitro.
METHODS: Mouse BMSCs were isolated and induced by endothelial-induced fluid to differentiate into endothelial cells. In addition, isolated mouse BMSCs were induced in these epithelial induction media for 3 weeks: a simple epithelial induction medium; epithelial induction medium plus 10 μg/L transforming growth factor β1. Non-induced mouse BMSCs were used as negative controls and the alveolar epithelium as a positive control.
RESULTS AND CONCLUSION: After cultured in the epithelial induction medium for 3 weeks, some cells changed from the spindle cells into a typical pebble-like epithelial cell. 60% induced cells expressed epithelial cell marker pan-CK, and RT-PCR showed that differentiated epithelial cells expressed cell-specific signs CK18. There was no CK18 expression in non-induced mouse BMSCs. Mouse BMSCs in the epithelial induction medium for 24 hours, typical vascular network structure appeared. vWF immunofluorescence staining showed that about 70% of cells were positive, and RT-PCR showed that differentiated cells expressed CD31, vWF, and CD34. It is indicated that BMSCs has in vitro cross-mesodermal multilineage differentiation ability.