Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7421-7424.doi: 10.3969/j.issn.1673-8225.2011.40.003

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Expression of osteoprotegerin gene mediated with liposome in rat bone marrow stromal cells

Zhao Ji-hua1, Li Qi-jia1, Guo Jing1, Wang Zhi-qiang2   

  1. 1Laboratory Center, Hebei United University, Tangshan  063000, Hebei Province, China; 2Department of Orthopaedics, Affiliated Hospital of Hebei United University, Tangshan  063000, Hebei Province, China
  • Received:2011-04-11 Revised:2011-06-22 Online:2011-10-01 Published:2011-10-01
  • Contact: Li Qi-jia, Professor, Laboratory Center, Hebei United University, Tangshan 063000, Hebei Province, China qjl1222@hotmail.com
  • About author:Zhao Ji-hua★, Studying for master’s degree, Laboratory Center, Hebei United University, Tangshan 063000, Hebei Province, China wxjhxz@sina.com
  • Supported by:

    the Foundation of Department of Human Resources of Hebei Province, No. 200911*

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Abstract:

BACKGROUND: Genetically modified seed cells for bone tissue engineering can improve the ability to repair bone defects.
OBJECTIVE: To detect the expression of osteoprotegerin (OPG) in rat transfected bone marrow stromal cells (BMSCs).
METHODS: BMSCs were aseptically obtained from the femur and tibia of 4-week-old SD rats and cultured in vitro. BMSCs were divided into three groups: plasmid group, blank plasmid group and non-transfection group.
RESULTS AND CONCLUSION: After transfection, green fluorescent protein was observed in BMSCs under the laser scanning confocal microscope. There was obvious mark at 1 200 bp in plasmid group, but there was no expression of OPG mRNA in blank plasmid group and non-transfection group. The OPG protein was positive in BMSCs detected by immunocytochemistry examination. There were no significant difference in cell proliferation in each group (P > 0.05). plRES2- EGFP-OPG plasmid transfected BMSCs can express exogenous OPG and proteins. 

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