Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7416-7420.doi: 10.3969/j.issn.1673-8225.2011.40.002

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Human heme oxygenase-1 improves the survival of bone marrow mesenchymal stem cells in ischemic heart

Zeng Bin, Yi Xin, Ma Le-le   

  1. Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan  430060, Hubei Province, China
  • Received:2011-04-12 Revised:2011-08-16 Online:2011-10-01 Published:2011-10-01
  • About author:Zeng Bin☆, Doctor, Attending physician, Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China zengbinwhu@yahoo.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30900609*

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Abstract:

BACKGROUND: Cardiac function is limited by the great amount of dead transplanted cells following myocardial infarction (AMI) treatment with bone marrow mesenchymal stem cells (BMSCs) transplantation.
OBJECTIVE: To investigate the effects of human heme oxygenase-1 (HO-1) on the survival of BMSCs in the heart after AMI.
METHODS: BMSCs were isolated, cultured and proliferated in vitro, transfected with Adv-hHO-1 and Adv-GFP, and then labeled with DAPI before transplantation. At 1 hour after left coronary artery ligation, DAPI-HO-1-BMSCs or DAPI-GFP-BMSCs were directly injected into the border of infracted cardiac region in rats. An equal volume of PBS was injected into the control group.
RESULTS AND CONCLUSION: The expression of hHO-1 mRNA, vascular endothelial growth factor, basic fibroblast growth factor, hepatocyte growth factor in the border of infracted cardiac region treated with HO-1-BMSCs were higher than those treated with GFP-BMSCs and PBS (P < 0.01), and the number of BMSCs and capillary vessels in AMI heart treated with HO-1-BMSCs was significantly higher than those treated with GFP-BMSCs and PBS (P < 0.05, P < 0.01). The heart function and remolding of the hearts treated with HO-1-BMSCs was better than those treated with GFP-BMSCs and PBS (P < 0.01). The infarct area in the HO-1-BMSCs group was also smaller than that in the GFP-BMSCs and PBS groups (P < 0.01). HO-1 enhances the survival of BMSCs in the AMI heart, which cooperates with BMSCs to inhibit ventricular remodeling and improve the cardiac function.

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