Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7411-7415.doi: 10.3969/j.issn.1673-8225.2011.40.001

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Effect of vibration stress stimulation on bone marrow stem cells for repairing rabbit bone defects

Zhang Lu, Zhao Wen-zhi, He Sheng-wei, Fang Xu, Mi Li-dong, Sun Xue-gang, Du Guang-yu, Sun Chuan-xiu   

  1. Department of Orthopedics, North Hospital, Second Affiliated Hospital of Dalian Medical University, Dalian  116023, Liaoning Province, China
  • Received:2011-04-01 Revised:2011-05-19 Online:2011-10-01 Published:2011-10-01
  • Contact: Zhao Wen-zhi, Doctor, Professor, Chief physician, Master’s supervisor, Department of Orthopedics, North Hospital, Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China drzwz@163.com
  • About author:Zhang Lu★, Master, Attending physician, Department of Orthopedics, North Hospital, Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China andrew76aa@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30970708*

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Abstract:

BACKGROUND: Mechanical factors can regulate and induce the differentiation of bone marrow mesenchymal stem cells (BMSCs) into bone cells, and increase the differentiation efficiency.
OBJECTIVE: To investigate the effect of vibration stress stimulation on the osteogenic differentiation ability of BMSCs in the bone defect microenvironment.
METHODS: Twenty-four rabbits were randomized into three groups: bone matrix gelatin (BMG), BMG+BMSCs, vibration+BMG+BMSCs groups. Humeral bone defect models were established in all the rabbits. Vibration stress stimuli were applied to live animals with 0.3 G acceleration, 25 Hz, sine wave type, 1 once a day, 30 minutes once, for 4 weeks.
RESULTS AND CONCLUSION: Gross observation display callus growth good in the vibration group, histological sections showed that the amount of new bone was large in the vibration group, and a large number of osteoblasts, bone formation and bone ends connected were found in the vibration group. mRNA expression of RUNX2 and type Ⅰ collagen was significantly higher in the vibration group than in the non vibration groups. Vibration can promote BMSCs, up-regulate RUNX2 gene, type Ⅰ collagen gene thus speeding up the repair of bone defects.

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