Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6691-6695.doi: 10.3969/j.issn.1673-8225.2011.36.010

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Flow-cytometric method for observing the effects of pulsed electromagnetic fields on the growth of rat bone marrow mesenchymal stem cells

Huang Zhao, Su Wei, Cui Xiang-rong, Qin Wan-an   

  1. Department of Trauma Osteology and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-06-24 Revised:2011-08-09 Online:2011-09-03 Published:2011-09-03
  • Contact: Su Wei, Professor, Chief physician, Department of Trauma Osteology and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China gxsuwei@163.com
  • About author:Huang Zhao★, Studying for master’s degree, Department of Trauma Osteology and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China gxmu@stemcell8.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30860078*; Scientific Research Program of Education Department of Guangxi Zhuang Autonomous Region, No. 200710MS021*; Scientific Research Fund of the First Affiliated Hospital of Guangxi Medical University*

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Abstract:

BACKGROUND: Compared with the morphology, DNA electrophoresis and other methods, flow-cytometric method has more advantages on the detection of cell phenotype, proliferation rate and cell cycle of rat bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To observe and discuss the effect of specific pulsed electromagnetic fields stimulation on the proliferation and cell cycle of rat BMSCs with the flow-cytometric method (FCM).
METHODS: Rat BMSCs were exposed to pulsed electromagnetic fields (frequency for 1 kHz, magnetic flux density for 0.05 mT, power density for 5 mW/cm2). BMSCs without exposure to pulsed electromagnetic fields were used as controls. Expression of CD29, CD31, CD44 CD45, CD105 were detect in the control group. The proliferation rate and cell cycle of passage 3 cells were detected at 3, 6, 9, 12 days.
RESULTS AND CONCLUSION: CD29, CD44, CD105 in the 3rd passage non-stimulated BMSCs was positively expressed and the CD31, CD45 was negatively expressed (P < 0.05). The survival rate of passage 3 BMSCs and percentage of S phase following intervention of pulsed electromagnetic fields were greater than those in the control group (P < 0.05). These results indicated that the specific pulsed electromagnetic fields can promote the growth and proliferation of BMSCs to some extent.

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