Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6696-6700.doi: 10.3969/j.issn.1673-8225.2011.36.011

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pcDNA3.1-osteogenic growth polypeptide eukaryotic expression vector in bone marrow mesenchymal stem cells

An Gang, Lü Song-cen, Guo Ya-shan, Xue Zhen, Deng Qiu-kui   

  1. Department of Orthopaedics, Second Affiliated Hospital, Harbin Medical University, Harbin  150086, Heilongjiang Province, China
  • Received:2011-03-19 Revised:2011-05-30 Online:2011-09-03 Published:2011-09-03
  • Contact: Lü Song-cen, Doctor, Professor, Department of Orthopaedics, Second Affiliated Hospital, Harbin Medical University, Harbin 150086, Heilongjiang Province, China lv226918@163.com
  • About author:An Gang★, Master, Physician, Department of Orthopaedics, Second Affiliated Hospital, Harbin Medical University, Harbin 150086, Heilongjiang Province, China abc7231719@163.com Guo Ya-shan★, Master, Attending physician, Department of Orthopaedics, Second Affiliated Hospital, Harbin Medical University, Harbin 150086, Heilongjiang Province, China guoyashan1972@163.com An Gang and Guo Ya-shan contributed equally to this study.
  • Supported by:

    the Returned Students Fund of Heilongjiang Province, No. LC04C03*;the Youth Scientific Research Fund of Harbin City, Training Reserve Leader of Subject Fund, the Returned Students Fund Project, No. 2005AFLXJ016*

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Abstract:

BACKGROUND: Osteogenic growth polypeptide (OGP) had clear effect on promoting osteoblast proliferation, differentiation and mature.
OBJECTIVE: To explore the expression of OGP gene, which was transfected into rabbit bone marrow mesenchymal stem cells (BMSCs) and to evaluate the effects of OGP on differentiation of rabbit BMSCs.
METHODS: pcDNA3.1-OGP was constructed using gene cloning and recombination techniques. Rabbit BMSCs were transfected with pcDNA3.1-OGP mediated by lipofectamine 2000. The transfection positive cell clones were selected with G418. The expression of OGP gene was detected using reverse transcription-polymerase chain reaction analysis on an mRNA level. Differentiation of pcDNA3.1-OGP transfected BMSCs into osteoblast lineage was observed.
RESULTS AND CONCLUSION: The pcDNA3.1-OGP plasmid was constructed successful and OGP expression was detected in rabbit BMSCs. Hydroxyproline content was increased, and alkaline phosphatase activity was also increased. These indicate that pcDNA3.1-OGP transfected BMSCs expressed OGP, and could differentiate into osteoblast lineage.

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