Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6687-6690.doi: 10.3969/j.issn.1673-8225.2011.36.009

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Culture of rat bone marrow mesenchymal stem cells by using adherent method and shortening the time of trypsin digestion

Li Shu-fa1, Zhang Min2   

  1. 1Department of Endocrinology, 2Zhonghua Community Health Center, the First People’s Hospital of Guiyang, Guiyang  550002, Guizhou Province, China
  • Received:2011-01-13 Revised:2011-04-10 Online:2011-09-03 Published:2011-09-03
  • Contact: Zhang Min, Attending physician, Zhonghua Community Health Center, the First People’s Hospital of Guiyang, Guiyang 550002, Guizhou Province, China 864769653@qq.com
  • About author:Li Shu-fa☆, Doctor, Associate chief physician, Department of Endocrinology, the First People’s Hospital of Guiyang, Guiyang 550002, Guizhou Province, China shufali@163.com

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Abstract:

BACKGROUND: There is no uniform standard for bone marrow mesenchymal stem cells (BMSCs) isolation, purification, culture and expansion, and no specific marker for BMSCs identification.
OBJECTIVE: To investigate the different isolation and culture methods of BMSCs.
METHODS: Rat BMSCs were isolated and cultured by the method of frequent replacement of culture medium at early stage, controlling trypsin digestion time and plastic adherent culture. The cultured cells were induced under different conditions. The differentiated adipoccytes, osteoblasts and chondrocytes were observed.
RESULTS AND CONCLUSION: Purified BMSCs can be obtained at the third generation with this isolation and culture method. The cultured cells have good proliferative potential. BMSCs can differentiate to adipocytes, osteoblasts and chondrocytes under certain conditions.

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