Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 2034-2037.doi: 10.3969/j.issn.1673-8225.2011.11.033

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Expression of cyclooxygenase-2 and inducible nitric oxide synthase in primary knee osteoarthritis interfered by Tougu Xiaotong granules

Liu Bo-ling1, Liang Gui-qing1, Liu Xian-xiang2, Wu Guang-wen2, Li Xi-hai2   

  1. 1Department of Orthopaedics, the Second Hospital of Fuzhou, Fuzhou  350007, Fujian Province, China
    2Academy of Integrated Traditional Chinese and Western Medicine, Fujian College of Traditional Chinese Medicine, Fuzhou  350108, Fujian Province, China
  • Received:2010-10-26 Revised:2011-01-06 Online:2011-03-12 Published:2011-03-12
  • About author:Liu Bo-ling☆, Doctor, Attending physician, Department of Orthopedics, the Second Hospital of Fuzhou, Fuzhou 350007, Fujian Province, China liuboling2000@126. com
  • Supported by:

    Program for Youth Talents in Fujian Province, No. 2007F3121*; Senile Disease Key Laboratory Open Topic of Fujian Province Integrated Traditional Chinese and Western Medicine, No. CKJ2008057, 2008J1004-20*

Abstract:

BACKGROUND: Mornda root, radix paeoniae alba from Hangzhou of China, zhongjiefeng, szechwan lovage rhizome composed of Tougu Xiaotong granule can effectively improve the symptoms of knee osteoarthritis (KOA), but the mechanism of it remains unclear.
OBJECTIVE: To observe the effect of Tougu Xiaotong granule on primary knee osteoarthritis cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression.
METHODS: A total of 90 New Zealand White rabbits were randomly divided into 6 groups: normal group, model group, Tougu Xiaotong granule low-dosage group, Tougu Xiaotong granule midst-dose group, Tougu Xiaotong granule high-dose group, and Zhuangguguanjie pill group. In addition to the normal group, osteoarthritis model was established by improved Hulth method in the other groups. Tougu Xiaotong granule low-dosage group, Tougu Xiaotong granule midst-dose group, Tougu Xiaotong granule high-dose group, and Zhuangguguanjie pill group was based on modeling treated with containing 5, 10, 20 g Tougu Xiaotong granule or Zhuangguguanjie aqueous solution of 10 g pill at every day, respectively. After 4 weeks, the level of nitrogen monoxidum (NO), NOS, and prostaglandin E2 (PGE2) in rabbit synovial fluid, articular cartilage, iNOS and COX-2 in synovium tissue was detected.
RESULTS AND CONCLUSION: In the early stage of osteoarthritis, the level of NO, NOS, PGE2, and the expression of COX-2, iNOS in synovium and cartilage were higher than that in normal group. Tougu Xiaotong granule and Zhuangguguanjie pill can reduce the expression of NO, NOS, PGE2, and expression of COX-2 and iNOS in synovium and cartilage. Tougu Xiaotong granule midst-dose group and high-dose group have the most significant effects. It is indicated that Tougu Xiaotong granule can inhibit degradation of cartilage, release clinical symptoms to protect articular cartilage, prevent and cure osteoarthritis by COX-2 and iNOS expression.

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