Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 2038-2041.doi: 10.3969/j.issn.1673-8225.2011.11.034

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Effect of Jingguishenqi pill on expression of apoptosis-related protein Bcl-2 and Bax in male mouse with kidney yang deficiency

Liu He-liang1, Chen Chang-sheng2, Qin Jun1, Yang Bo1, Qin Wei-jun1, Zhang Geng1, Yuan Jian-lin1   

  1. 1Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi’an  710033, Shaanxi Province, China
    2Room of Military Preventive Medicine, Department of Health Statistics, Fourth Military Medical University, Xi’an  710033, Shaanxi Province, China
  • Received:2010-09-16 Revised:2010-10-30 Online:2011-03-12 Published:2011-03-12
  • Contact: Chen Chang-sheng, Doctor, Professor, Room of Military Preventive Medicine, Department of Health Statistics, Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China chencs@fmmu.edu.cn Yuan Jian-lin, Doctor, Professor, Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China ccscbr@sina.com
  • About author:Liu He-liang☆, Doctor, Associate chief physician, Associate professor, Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China liuhel@fmmu.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30772683*; the Tackle Key Program in Science and Technology of Shaanxi Province, No. 2008K04-02*

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Abstract:

BACKGROUND: Kidney-deficiency causes sterility. Jingguishenqi pill is a classical Chinese traditional medicine for treating kidney yang deficiency, but its mechanism remains poorly understood.
OBJECTIVE: To explore the effect of Jingguishenqi pill on expression of Bcl-2 and Bax in male mice permatogenic cells with kidney yang deficiency.
METHODS: Totally 100 mature male rats were divided into 5 groups randomly: blank control group (normal group), model group, Jingguishenqi pill with low dosage group, middle dosage group, high dosage group. Except the normal group, the others were made into model of kidney-yang deficiency by injecting hydrocortisone. After the model preparation, the mice in the normal and model groups were fed with distilled waters, and those in the Jingguishenqi pill with different dosage groups were fed with the same dose of medicine (0.625, 1.250 and 2.500 g/kg) for 30 days continuously. Then, the weight of the left testis was measured, and the blood level of testosterone, and the sperm density, survival rate were examined. Then the expression of Bcl-2 and Bax in spermatogenic cells were investigated with the immunohistochemistry and image analysis.
RESULTS AND CONCLUSION: The weight of left testis, blood level of testosterone, sperm density, sperm survival rate in the model group were decreased than that in the control, Jingguishenqi pill with low dosage group, middle dosage group, high dosage group (P < 0.05). The number of cells of expressing Bcl-2 and the intensity of its expression in the model group were lower than that in other four groups (P < 0.05). The number of cells of expressing Bax and the intensity of its expression in the model group were higher than that in other four groups (P < 0.05). Jingguishenqi pill can promote the expression of Bcl-2 but reduce Bax expression, which exerts a certain therapeutic effect on the kidney-yang deficiency in rats.

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