Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 2030-2033.doi: 10.3969/j.issn.1673-8225.2011.11.032

Previous Articles     Next Articles

Effect of epimedium and bone morphogenetic protein-2 on fracture healing in rabbits

Wang Xin-wen, He Ai-yong, Cai Yu-zhong, Yan Zhen-sheng   

  1. First Department of Orthopaedics, the Second Xiangya Hospital of Central South University, Changsha  410011, Hunan Province, China
  • Received:2010-10-22 Revised:2010-11-06 Online:2011-03-12 Published:2011-03-12
  • Contact: He Ai-yong, Professor, Doctor, Doctoral supervisor, First Department of Orthopaedics, the Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China heaiy57@yahoo.com.cn
  • About author:Wang Xin-wen★, Master, First Department of Orthopaedics, the Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China wangxinwen139@163.com
  • Supported by:

    Scientific Research Fund of Hunan Provincial Health Department, No. 132009-023*

PDF

568

Abstract:

BACKGROUND: Epimedium is a traditional Chinese medicine for osteoporosis prevention and treatment, but the treatment on fracture healing is still lack of basis.
OBJECTIVE: To investigate the effect of epimedium on fracture healing in rabbits. 
METHODS: The models with 3mm fracture were established in half radius in rabbits, and were randomly divided into blank group, control group, and experimental group. The blank group was treated with normal saline by gastric perfusion and injected with normal saline in the part bone fracture. The experimental group was treated with epimedium by gastric perfusion and injected with normal saline. The control group was treated with normal saline by gastric perfusion and injected with bone morphogenetic protein-2 (BMP-2) in the part of bone fracture. At 2, 4, 8 weeks post-surgery, 4 rabbits were harvested to evaluate the degree of fracture healing according to X-ray radiography, bone density test, biomechanical test (only 8 weeks), and tissue section, etc.
RESULTS AND CONCLUSION: X-ray radiograph showed that the experimental and control groups are better than the blank group in callus formation, remodeling, and recanalization of medullary cavity at each period. Bone densities in experimental group were prior to that in blank group (P < 0.05). There was no significant difference between the experimental group and control group (P > 0.05). Biomechanical properties of experimental group were better than that in the blank group (P < 0.05), the values of control group was between the other groups. The formation period of collagen fiber, cartilage tissue, bone trabecula, and bone matrix was earlier in the experimental and control groups compared with the blank group. There was no significant difference between the experimental and control groups. It is indicated that epimedium can promote fracture healing in rabbits.

CLC Number: