Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 1079-1082.doi: 10.3969/j.issn.1673-8225.2011.06.028

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Non-enzymatic nitric oxide induced by ultrasound mediated microbubble destruction

Li Peng1, Tong Jia-yi1, Feng Yi1, Yang Fang2, Ma Gen-shan1, Chen Long1, Song Jia-xian1   

  1. 1Cardiovascular Research Institute, Medical College, Southeast University, Nanjing  210009, Jiangsu Province, China
    2Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing  210009, Jiangsu Province, China
  • Received:2010-09-06 Revised:2010-11-15 Online:2011-02-05 Published:2011-02-05
  • Contact: Feng Yi, Doctor, Chief physician, Professor, Cardiovascular Research Institute, Medical College, Southeast University, Nanjing 210009, Jiangsu Province, China
  • About author:Li Peng★, Studying for master’s degree, Cardiovascular Research Institute, Medical College, Southeast University, Nanjing 210009, Jiangsu Province, China
  • Supported by:

    the Special Scientific Research Foundation of Doctor Subject Center of Higher Learning School of Ministry of Education in 2009, No. 20090092120059*; the Advanced Research Foundation of the National Natural Science Foundation for Southeast University in 2009, No. KJ2009386*

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Abstract:

BACKGROUND: Nitric oxide is one of the most important gas signaling molecule. Nitric oxide could be synthesized without nitric oxide synthetase, non-enzymatic nitric oxide, which might be one of the mechanisms of some biological effects.
OBJECTIVE: To observe whether ultrasound mediated microbubbles could enhance the product by non-enzymatic pathway.
METHODS: Laevo-arginine (L-Arg) was mixed with hydrogen peroxide (H2O2) by the concentration rates of 1: 1, 10: 1, 10: 0.1,  1: 10, which were irradiated with ultrasound for 60 seconds (frequency: 1 MHz, output power: 0.5, 1.0, 1.5 W/cm2). The optimal concentration of L-Arg and H2O2 and optimal output power of ultrasound were discussed and identified. This study set up four groups, and received respectively ultrasound mediated microbubbles, simple ultrasound and microbubbles. Blank control group did not receive any intervention. Formation amount of nitric oxide was compared among groups.
RESULTS AND CONCLUSION: In vitro experiment, the optimal concentration ratio of L-Arg and H2O2 was 10: 1, and 1.5 W/cm2 was the optimal ultrasound value. The content of nitric oxide was more in the ultrasound mediated microbubbles group than the simple ultrasound group (P < 0.01). The content of nitric oxide was better in the simple ultrasound group compared with blank control group (P < 0.01). No significant difference in the content of nitric oxide was detected between microbubble and blank control groups. Results suggested that ultrasound mediated microbubbles can enhance non-enzymatic synthesis of nitric oxide.

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