Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 1057-1060.doi: 10.3969/j.issn.1673-8225.2011.06.023

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Experimental research on adipose-derived stromal cells in tissue engineering bone for the repair of canine mandibular bone defects

Li Xiao-yu1, Yao Jin-feng2, Liu Zheng-hua1, Cai Jian-bo1   

  1. 1Department of Oral and Maxillofacial Surgery, Shenzhen Baoan People's Hospital, Shenzhen  518101, Guangdong Province, China
    2Department of Stomatology, The Second People’s Hospital of Shenzhen, Shenzhen  518035, Guangdong Province, China
  • Received:2010-09-09 Revised:2010-12-10 Online:2011-02-05 Published:2011-02-05
  • Contact: Cai Jian-bo, Associate chief physician, Department of Oral and Maxillofacial Surgery, Shenzhen Baoan People's Hospital, Shenzhen 518101, Guangdong Province, China caijianbo60@163.com
  • About author:Li Xiao-yu☆, Doctor, Attending physician, Department of Oral and Maxillofacial Surgery, Shenzhen Baoan People's Hospital, Shenzhen 518101, Guangdong Province, China xiaoyuli204@163.com

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Abstract:

BACKGROUND: Previous studies demonstrated that only adipose-derived stromal cells (ADSCs) after osteogenic induction can be served as seeding cells for bone tissue engineering. However, the process of osteogenic induction is complicated, making the procedure expensive and elongating the culture time in vitro.
OBJECTIVE: To explore the feasibility of repairing the canine mandibular bone defects with tissue engineering method based on non-osteogenically induced ADSCs as seeding cells.
METHODS: The subcutaneous fat from the back of dogs aged 12 months were obtained, mononuclearcell was obtained by collagenase digestion method. The cultured 3rd passage cells and biphasic calcium phosphate (BCP) ceramics formed scaffold composite. 20 mm in length and 10 mm in height of box-like bone defects were prepared in both sides of canine mandibular; the teeth were extracted in defect region. The cell-scaffold composite and BCP ceramics scaffold were implanted in canine mandibular bone defects. The region without intervention served as control. The repair of bone defects was detected by histology, at 4 weeks and 8 weeks implantation.
RESULTS AND CONCLUSION: After 4 weeks scaffold implantation, part of scaffold materials was degraded; new bone was formed in defect region. The bone formation in BCP group was significantly less than that in cell-scaffold composite group, and formed a small amount of new bones and part of the new vessels. At 8 weeks scaffold implantation, more new bones were formed between BCP group and cell-scaffold composite group, which were widely distributed in the bone defect region. However, the new bones in BCP group were still significantly less than that in cell-scaffold composite group; there were significant differences (P < 0.01). It is indicated that ADSCs combine with BCP can promote bone formation in vivo. ADSCs-scaffold construct can promote bone formation in vivo. Non-osteogenically induced ADSCs can be used as seed cells to repair the mandibular bone defects by tissue engineering technology.

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