Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 1040-1043.doi: 10.3969/j.issn.1673-8225.2011.06.020

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Olfactory ensheathing cells transplantation combined with Du meridian electro-acupuncture for treatment of anterior spinal cord injury in rats 

Zhang Shu-lian, Yan Jin-yu, Li Jian-feng   

  1. Department of Rehabilitation, Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot  010030, Inner Mongolia Autonomous Region, China
  • Received:2010-09-09 Revised:2010-10-13 Online:2011-02-05 Published:2011-02-05
  • Contact: Li Jian-feng, Master, Department of Rehabilitation, Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010030, Inner Mongolia Autonomous Region, China jianfeng0471@163.com
  • About author:Zhang Shu-lian, Nurse-in-charge, Department of Rehabilitation, Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010030, Inner Mongolia Autonomous Region, China alian0471@163.com Yan Jin-yu,Chief physician, Professor, Department of Rehabilitation, Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010030, Inner Mongolia Autonomous Region, China pangziljf@yahoo.com.cn Zhang Shu-lian and Yan Jin-yu contributed equally to this paper.
  • Supported by:

    the National Natural Science Foundation of China, No.30860300*

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Abstract:

BACKGROUND: Electro-acupuncture has effects on rehabilitation of spinal cord injury, and cell therapy such as olfactory ensheathing cells (OECs) also exert effects in some patients’ rehabilitation. Whether both have synergistic effects remains poorly understood.
OBJECTIVE: To observe the effects of Du meridian electro-acupuncture and OECs transplantation on content of neurotrophic factor-3 and expression of P75NTR in anterior spinal cord injury rats. 
METHODS: A total of 40 adult rats were randomly divided into: control group, DU meridian electro-acupuncture group, OECs transplantation group, DU meridian electro-acupuncture + OECs transplantation group. We measured the content of neurotrophic factor-3 and expression of P75NTR by enzyme linked immunosorbent assay at 2 weeks after OECs transplantation and DU meridian electro-acupuncture treatment.
RESULTS AND CONCLUSION: The content of NT-3 was highest in DU meridian electro-acupuncture + OECs transplanted group compared with other groups (P < 0.05). There were P75NTR expressions in both OECs transplantation group and DU meridian electro-acupuncture + OECs transplantation group, and the expression was more in DU meridian electro-acupuncture + OECs transplantation group than the OECs transplantation group. These indicated that Du meridian electro-acupuncture and OECs transplantation can elevate the content of neurotrophic factor-3 in anterior spinal cord injury rats. Du meridian electro-acupuncture can improve the survival of transplanted OECs in the host.

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