Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 995-999.doi: 10.3969/j.issn.1673-8225.2011.06.011

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Effects of neurotrophic factors in umbilical cord blood plasma on CD34+ cell proliferation and differentiation

Zhang Li1, 2, Li Qian-ru2, Du Ying2, Yang Bo3, Li Guo-xi4, Hu Xiang4   

  1. 1Henan Province Red Cross Blood Center, Zhengzhou  450053, Henan Province, China
    2Department of Microbiology and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou  450001, Henan Province, China
    3Department of Neurosurgery, First Clinical Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    4Shenzhen Beike Cell Engineering Institute, Shenzhen  518000, Guangdong Province, China
  • Received:2010-09-11 Revised:2010-11-09 Online:2011-02-05 Published:2011-02-05
  • Contact: Du Ying, Doctor, Professor, Master’s supervisor, Department of Microbiology and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou 450001, Henan Province, China duying@zzu.edu.cn
  • About author:Zhang Li★, Studying for master’s degree, Attending physician, Henan Province Red Cross Blood Center, Zhengzhou 450053, Henan Province, China; Department of Microbiology and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou 450001, Henan Province, China lilyzhl79@163.com Li Qian-ru, Doctor, Associate professor, Department of Microbiology and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou 450001, Henan Province, China Zhang Li and Li Qian-ru contributed equally to this article.

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Abstract:

BACKGROUND: Further studies are needed to understand if the neurotrophic factors in human umbilical cord blood plasma have the promoting effect on neural regeneration and repair.
OBJECTIVE: To investigate the component of neurotrophic factors in umbilical cord blood plasma, observe its application in neural regeneration and repair.
METHODS: The difference was compared between the human umbilical cord blood plasma (HUCBP) and peripheral venous blood plasma (PVBP) from healthy voluntary womenfolk using human antibody array. The CD34+ cells were sorted from human umbilical cord blood using immunomagnetic beads. Following cultivating in vitro and counting, proliferative ability of CD34+ cells was observed. Cell differentiation was observed using immunohistochemical method.
RESULTS AND CONCLUSION: The concentrations of 31 kinds of protein molecules were significantly higher in HUCBP than PVBP. Of them, 10 kinds of molecules thereinto have significant potential of nerve regeneration and plasticity, including FGF4, Frizzled-3, IL-3, RAGE, CRIM-1, Neuritin, Neuropilin-2, Neurturin, SFRP-3 and Tomoregulin-1. HUCBP may promote the CD34+ cells proliferation in cell culture medium and differentiation into nerve cells. 

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