Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 980-984.doi: 10.3969/j.issn.1673-8225.2011.06.008

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Mixed culture of human amnion-derived mesenchymal stem cells from different sources

Wang Zhao-fu1, Jiao Hong-liang1,2,3, Li Jian-bin1, Shan Hong1, Zheng Wen-di4, Gu Chen-xi4, Du Ying5, Guan Fang-xia6, Yang Bo2,3   

  1. 1Henan Provincial Red Cross Blood Center, Zhengzhou  450014, Henan Province, China
    2Department of Neurosurgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou  450052, Henan Province, China
    3Open and Key Laboratory of Clinical Medicine of Henan Colleges and Universities, Zhengzhou  450052, Henan Province, China
    4Department of Clinical Medicine, Zhengzhou University, Zhengzhou  450001, Henan Province, China
    5Department of Microbiology and Immunology, Basic Medicine School of Zhengzhou University, Zhengzhou  450052, Henan Province, China
    6Department of Biomedical Engineering, Zhengzhou University, Zhengzhou  450052, Henan Province, China
  • Received:2010-11-20 Revised:2010-12-20 Online:2011-02-05 Published:2011-02-05
  • Contact: Guan Fang-xia, Professor, Department of Biomedical Engineering, Zhengzhou University, Zhengzhou 450052, Henan Province, China guanfangxia@126.com Yang Bo, Professor, Doctoral supervisor, Department of Neurosurgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open and Key Laboratory of Clinical Medicine of Henan Colleges and Universities, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Wang Zhao-fu, Associate professor, Henan Provincial Red Cross Blood Center, Zhengzhou University, Zhengzhou 450014, Henan Province, China
  • Supported by:

    the Key Subjects of Zhengzhou University*; Henan Innovation Talent Project of Medical Science, No. 2005018*

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Abstract:

BACKGROUND: Human amnion-derived mesenchymal stem cells (HAMs) can be induced to differentiate into neuron-like cells, but the number of stem cells in the training process is insufficient.
OBJECTIVE: Observed HAMs the growth of mixed culture, and induced HAMs to the nerve-like cells by basic fibroblast growth factor (bFGF) to obtain a sufficient number of HAMs.
METHODS: Amnions from two placentas were separated and cultured to obtain HAMs, which were induced differentiation by adding bFGF. When cells were at the 3rd passage, cultured 6 HAMs (half dose) were randomly taken for the A group, 6 HAMs (half dose) were taken for the B group, the remaining half of the amount of the A, B group HAMs mixed as C group. Cell densities of 3 groups were 1.0×107/L. The cells proliferations of 3 groups were compared by live cell count and MTT colorimetric. The expressions of GFAP, neuronspecific enolase (NSE) and nestin were detected by immunohistochemistry.
RESULTS AND CONCLUSION: Mixing HAMs have mutual effect on the proliferation. HAMs have a strong retrospective, which can express GFAP, NSE, and nestin induced by bFGF.

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