Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 976-979.doi: 10.3969/j.issn.1673-8225.2011.06.007

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Steroid effects on neurotransmitter expressions in bone marrow mesenchymal stem cells of rabbits

Wang Yi-sheng1, Zhang Xin1, Zhang Zhen1, Zhao Guo-qiang2, Li Yue-bai3   

  1. 1Departmemt of Orthopaedic Surgery, First Affiliated Hospital, Zhengzhou University, Open Laboratory of Unode Science of Clinical Medicine of Henan Province, Zhengzhou  450052, Henan Province, China
    2Department of Biochemistry, Basic Medical College, Zhengzhou University, Zhengzhou  450001, Henan Province, China
    3Department of Microbiology & Immunology, Basic Medical College, Zhengzhou University, Zhengzhou  450001, Henan Province, China
  • Received:2010-11-15 Revised:2010-12-12 Online:2011-02-05 Published:2011-02-05
  • About author:Wang Yi-sheng, Professor, Chief physician, Doctoral supervisor, Departmemt of Orthopaedic Surgery, First Affiliated Hospital, Zhengzhou University, Open Laboratory of Unode Science of Clinical Medicine of Henan Province, Zhengzhou 450052, Henan Province, China wangyisheng@zzu.edu.cn
  • Supported by:

    the Key Science and Technology Project of Henan Province in 2008, No. 082102310020*; the “211 Engineering” Third-Stage Construction Program of Zhengzhou University*

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Abstract:

BACKGROUND: The precise etiopathogenesis of steroid-induced necrosis of femoral head remains unclear. Studies have demonstrated that the differentiation of bone marrow mesenchymal stem cells (BMSCs) was regulated by nervous system in various ways. The regulation mechanism could be affected by steroid to induce osteonecrosis.
OBJECTIVE: To observe the changes in neurotransmitter or its receptor mRNA expression in the BMSCs under the steroid condition.
METHODS: The rabbit BMSCs were cultivated and isolated by adherence and density gradient centrifugation. The 3rd passage of BMSCs was randomly divided into two groups. In the experimental group, the cells were treated with 10-7 mol/L dexamethasone. In the control group, the cells were normally treated without dexamethasone. The mRNA expressions of nerve growth factor (NGF),  fibroblast growth factor (FGF), calcitonin gene-related peptide receptor (CGRPR), vasoactive intestinal peptide receptor (VIPR), substance P receptor (SPR) and peroxisom proliferator-activeted receptor-γ (PPARγ) of BMSCs were detected on days 4, 7, 11, 15.
RESULTS AND CONCLUSION: The expressions of NGF mRNA, FGF mRNA, CGRPR mRNA, VIPR mRNA and SPR mRNA in the experimental group were significantly lower than that in the control group, but the expression of PPARγ mRNA increased significantly (P < 0.01). There were no significantly differences compared among the experimental groups at various time points  (P > 0.05). Results suggested that high-dose steriod could depress the expressions of the neurotransmitters or their receptors which could promote the processes of ossification or vascularization in BMSCs. This might be one of the mechanisms for the development of the steroid-induced necrosis of femoral head.

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