Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9307-.doi: 10.3969/j.issn.1673-8225.2010.49.043

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Induction of umbilical cord blood-derived mesenchymal stem cells differentiating into pancreatic islet β-like cells in vitro

Chi Zuo-hua1, Lu Yan2, Zhang Yuan2   

  1. 1Medical College of Guangdong Pharmaceutical University, Guangzhou   510310, Guangdong Province, China;   2Institute of Hematopathy, Medical College of Jinan University, Guangzhou   510632, Guangdong Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Zhang Yuan, Doctor, Investigator, Institute of Hematopathy, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China tzyuan@jnu.edu.cn
  • About author:Chi Zuo-hua☆, Doctor, Associate professor, Medical College of Guangdong Pharmaceutical University, Guangzhou 510310, Guangdong Province, China zhchi33@126.com

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Abstract:

BACKGROUND: At present, there are many reports regarding the differentiation of bone marrow-derived mesenchymal stem cells or pancreatic gland stem cells into pancreatic islet β-like cells. But little is about umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) differentiation into pancreatic islet β-like cells in vitro.
OBJECTIVE: To investigate whether UCBMSCs can differentiate into pancreatic islet β-like cells in vitro and the optimal inducing condition.
METHODS: UCB samples were obtained sterilely from healthy parturients. Nucleated cells were isolated by sedimentation with hydroxyethyl starch and MSCs were obtained by adherent method. Then purified UCBMSCs were induced with epidermal growth factor, β-mercaptoethanol, high glucose, Activin A and hepatocyte growth factor (HGF). Following cell morphology observation, induced cells were identified by insulin immunofluorescence. In addition, insulin secretion and glucose-stimulated insulin release were examined by chemiluminescence immunoassay.   
RESULTS AND CONCLUSION: After induction, many cells exhibited a round appearance and produced islet-like cell clusters. Immunofluorescence assay showed insulin positive in the treated cells. In addition, chemiluminescence immunoassay demonstrated low expression of insulin and secretion of insulin upon glucose challenge. UCBMSCs can differentiate into pancreatic islet β-like cells in vitro. 

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