Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9249-.doi: 10.3969/j.issn.1673-8225.2010.49.029

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Changes in the number of endothelial progenitor cells from peripheral blood in patients with coronary atherosclerotic heart disease before and after extracorporeal cardiac shock wave therapy

Cai Hong-yan, Wang Yu, Li Lin, Zhao Ling, Xiao Jian-ming, Cao Jing-ming, Yang Xi-yun, Yang Chao, Guo Tao   

  1. Department of Cardiology, the First Affiliated Hospital of Kunming Medical College, Kunming    650031, Yunnan Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Guo Tao, Master, Chief physician, Doctoral supervisor, Department of Cardiology, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China GuoTao20@hotmail.com
  • About author:Cai Hong-yan☆, Studying for doctorate, Attending physician, Department of Cardiology, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China hyflykm@sina.com.cn
  • Supported by:

    Social Research Project of Yunnan Province, No.2006SG10*; Social Development Program of Yunnan Province, No.2008CD007*

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Abstract:

BACKGROUND: The mechanism underlying the effect of extracorporeal cardiac shock wave therapy on endothelial progenitor cells (EPCs) from peripheral blood in patients with coronary atherosclerotic heart disease (CAHD) is poorly understood.
OBJECTIVE: To observe the effects of cardiac shock wave therapy on the proliferation and the differentiation of EPCs from peripheral blood in CAHD patients.
METHODS: A total of 25 CAHD patients undergoing extracorporeal cardiac shock wave therapy were selected, and another 10 patients who did not receive extracorporeal cardiac shock wave therapy served as controls. Mononuclear cells obtained from peripheral blood were cultured in EGM-2-MV medium. Cell morphology and the number of colonies formed were observed and analyzed. After 7 days of culture, adherent cells were analyzed and counted. Whether EPCs differentiated or not was identified by laser confocal microscopy.
RESULTS AND CONCLUSION: The number of EPCs from peripheral blood and colonies formed were significantly increased (P < 0.05) compared with the control group. It is indicated that extracorporeal cardiac shock wave therapy can promote the proliferation and differentiation of EPCs from peripheral blood into endothelial cell lineage in CAHD patients.

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