Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9244-.doi: 10.3969/j.issn.1673-8225.2010.49.028

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Application of rabbit adipose-derived stem cells to the expanded flap with congestion

Liang Jiu-long 1, 2, Liu Xiao-yan2, Tao Kai2, Li Xia2   

  1. 1Second Military Medical University of Chinese PLA, Shanghai  200433, China; 2Department of Plastic Surgery, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang 110016, Liaoning Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Liu Xiao-yan, Master, Chief physician, Professor, Department of Plastic Surgery, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang 110016, Liaoning Province, China kk-lxy@sohu.com
  • About author:Liang Jiu-long☆, Studying for doctorate, Physician, Second Military Medical University of Chinese PLA, Shanghai 200433, China; Department of Plastic Surgery, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang 110016, Liaoning Province, China liangjiulong@sohu.com
  • Supported by:

    the Natural Science Foundation of Liaoning Province, No.20071041*

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Abstract:

BACKGROUND: To expand skin flap congestion is a difficulty in the plastic surgery. Previous methods are limited in application. Adipose-derived stem cells (ADSCs) can prolong the length of survived expanded skin flap.
OBJECTIVE: To solve the problem of congestion in the expanded flap by rabbit ADSCs.
METHODS: A total of 20 New Zealand rabbits were used to establish models of expanded flap congestion. 10 mL expandor was implanted in the skin of the back of the ear. Water was regularly injected. On day 20, the expandor was obtained to form expanded congestion flap. Autologous ADSCs were injected into one side of the congestion flap as experimental group. Saline was injected into another side as control group. At 2 weeks following injection, the survival of the flap was observed. Pathological sections were observed. Immunohistochemistry staining for CD34 was performed to quantitatively analyze vascularization.
RESULTS AND CONCLUSION: The survived length of flap was significantly longer in the experimental group than the control group (P < 0.01). Pathological sections demonstrated that the density of CD34 staining was significantly greater in the experimental group compared with the control group (P < 0.01). Results indicated that ADSCs can increase vascularization, improve congestion of the flap, and elevate the length in the expanded flap.

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