Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9239-.doi: 10.3969/j.issn.1673-8225.2010.49.027

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Differentiation of modified method cultured human dental pulp mesenchymal stem cells into neuron-like cells

Li Yuan 1, 2, Guan Fang-xia3, Li Ji4, Yang Bo 1,2, Hu Xiang 5,6, Du Ying7, Gu Chen-xi8, Lei Ning-jing 3, Wang Xiao-wei9   

  1. 1Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 2Open and Key Laboratory of Clinical Medicine at Henan Universities, Zhengzhou  450052, Henan Province, China; 3Department of Bioengineering, Zhengzhou University, Zhengzhou  450001, Henan Province, China; 4Department of Stomatology, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052 Henan Province, China; 5Jiangsu Public Technology Service Platform of Stem Cells and Biotherapy, Taizhou  225300, Jiangsu Province, China; 6Shenzhen Beike Cell Engineering Institute, Shenzhen  518000, Guangdong Province, China; 7Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 8Department of Clinical Medicine, Zhengzhou University, Zhengzhou  450001, Henan Province, China; 9College of Life Science, Hunan Normal University, Changsha  410081, Hunan Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Yang Bo, Master, Professor, Doctoral supervisor, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open and Key Laboratory of Clinical Medicine at Henan Universities, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Li Yuan☆, Studying for doctorate, Physician, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open and Key Laboratory of Clinical Medicine at Henan Universities, Zhengzhou 450052, Henan Province, China Liyuans889@Gmail.com Guan Fang-xia, Doctor, Professor, Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China guanfangxia@126.com Li Yuan and Guan Fang-xia contributed equally to this article
  • Supported by:

    the Zhengzhou University ‘211 project-phase Ⅲ’; the Jiangsu Public Technology Service Platform of Stem Cells and Biotherapy, No. BM2008146*

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Abstract:

BACKGROUND: The success rate of dental pulp stem cells (DPSCs) which isolated by the modified cultured method of tissue is elevated. However, few reports have concerned the difference in biological features between DPSCs and stem cells extracted by emzyme digestion method.
OBJECTIVE: To observe the difference in the differentiation of DPSCs isolated by the new method and isolated by traditional method into neuron-like cells.
METHODS: The specimen of the same tissue was randomly divided into two parts: one is isolated by the modified cultured method of tissue (A group), another is isolated by the traditional method (B group). Growth, antigen expression and differentiation of DPSCs into neuron-like cells were observed and compared using immunohistochemical method, flow cytometry and reverse transcription-polymerase chain reaction.
RESULTS AND CONCLUSION: There were no distinct changes in morphology expression, antigen ability, differentiation ability into neurons between A group and B group. However, A group had simple process and obtained a large quantity.

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