Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9216-.doi: 10.3969/j.issn.1673-8225.2010.49.022

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Erythroid lineage differentiation of K562 cells induced by gensingnoside Re

Lei Cui-rong1, Jiang Rong1, Wang Hong-ning1, He Xuan1, Zuo Guo-wei2, Chen Di-long1, Guan Tao3, Wang Jian-wei1   

  1. 1Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering,  Chongqing Medical University, Chongqing  400016, China; 2Key Laboratory of Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing  400016, China; 3Department of Physiology, Chongqing Medical University, Chongqing 400016, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Wang Jian-wei, Doctor, Professor, Doctoral supervisor, Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China wjwcq@yahoo.com.cn
  • About author:Lei Cui-rong★, Studying for master’s degree, Physician, Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China zhuozhi1014@163.com

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Abstract:

BACKGROUND: Gensingnosides can promote normal hematopoiesis and inhibit proliferation of leukemia cells. Gensingnoside Re as the main active ingredient in Ginsenosides has a variety of activities. However, Re induces leukemia cells differentiation and the mechanisms remain unclear.
OBJECTIVE: To investigate the effect of Gensingnoside Re on differentiation of chronic myelocytic leukemia K562 cells.
METHODS: The density of K562 cells at logarithmic phase was regulated to 7×108/L. Cells in the blank control group were routinely cultured in RPMI1640 medium. Cells in the Gensingnoside Re group were incubated in RPMI1640 medium containing Gensingnoside Re with the final concentration of 30, 60, 90, 120, 150 μmol/L. Wright’s staining, benzidine staining and hemoglobinometry were utilized to determine the characteristics of K562 cell differentiation into erythroid cells. Expressions of markers on the surface of K562 cells were determined by flow cytometry. 
RESULTS AND CONCLUSION: Gensingnoside Re 90 μmol/L could induce the K562 cell differentiation into erythroid cells. ① The morphological feature of K562 cells presented diminished volume, reduced nuclear diameter, abundant cytoplasm, and decreased karyoplasmic ratio. ②Hemoglobin in K562 cells induced by gensingnoside Re increased significantly in vitro. ③CD13 expressions on the surface of K562 cells increased.

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