Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9212-.doi: 10.3969/j.issn.1673-8225.2010.49.021

Previous Articles     Next Articles

Biological viability of rat bone marrow mesenchymal stem cells after the interference of astragalus mongholicus

Sun Li1, Jiao Bao-liang2, Wang Jing-chuan2, Wang Xin-sheng1   

  1. 1Experimental Center, Hebei North University, Zhangjiakou  075029, Hebei Province, China; 2First Clinical Medical School, Hebei North University, Zhangjiakou  075029, Hebei Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Wang Xin-sheng, Doctor, Professor, Master’s supervisor, Experimental Center, Hebei North University, Zhangjiakou 075029, Hebei Province, China wxinsheng1968@sina.com
  • About author:Sun Li, Senior laboratory technician, Experimental Center, Hebei North University, Zhangjiakou 075029, Hebei Province, China sunli11011@yahoo.com.cn
  • Supported by:

    the Doctor Foundation of Hebei North University

PDF

338

Abstract:

BACKGROUND: Astragalus mongholicus contributes to the differentiation of bone marrow mesenchymal stem cells (BMSCs) into neurons.
OBJECTIVE: To observe effects of astragalus mongholicus on viability of rat BMSCs.
METHODS: BMSCs of Sprague Dawley rats were isolated and cultured. The fourth passage of cells were treated with 50, 100,
200 g/L astragalus mongholicus for 5, 12, 24 hours respectively. BMSCs viability changes were observed by MTT assay after they were induced by astragalus mongholicus.
RESULTS AND CONCLUSION: Primarily cultured cells were closed to 80%-90% fusion status after 8-10 days culture and they could be passaged. The third generation had been purified on the whole. The results from MTT assay showed that the BMSCs viability would be increased with the increased concentration of astragalus mongholicus and the prolonged action time. Morphological changes in neural cells appeared. There were significant differences between groups (P < 0.05). These indicate that astragalus mongholicus can induce the differentiation of BMSCs and nourish cells.

CLC Number: