Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9208-.doi: 10.3969/j.issn.1673-8225.2010.49.020

Previous Articles     Next Articles

Effects of dendritic cells-cytokine-induced killer cells against leukemia K562 cell lines in vitro

Song Ying-ying, Su Rong-ying, Ai Li-mei   

  1. Department of Hematology, First Affiliated Hospital, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Ai Li-mei, Chief physician, Department of Hematology, First Affiliated Hospital, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China alm121001@yahoo.com.cn
  • About author:Song Ying-ying★, Studying for master’s degree, Department of Hematology, First Affiliated Hospital, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • Supported by:

    the Scientific Research Program of Department of Education of Liaoning Province, No. 2008390*; the Doctor Priming Foundation of Liaoning Medical University in 2009*

PDF

504

Abstract:

BACKGROUND: The combined application of cytokine-induced killer cells (CIK) and dendritic cells to treatment of malignant tumors will help to remove T cell incompetence of some cancer patients, and plays synergistic anti-tumor effects.
OBJECTIVE: To observe the effect of CIK cells co-cultured with dendritic cells against K562 cell lines.
METHODS: CIK and dendritic cells were induced by peripheral blood mononuclear cells of normal persons. Dendritic cells and CIK were co-cultured for 3 days as effector cells. Immunophenotype of cells was measured by flow cytometry. K562 served as target cells, and peripheral blood mononuclear cells, CIK, dendritic cells, dendritic cells-CIK as effector cells. The killing activity was assayed by MTT assay.
RESULTS AND CONCLUSION: With the increased ratio of effector to target, effector killing activity of each group also increased; for the same ratio, the killing activity of each group was different, of which dendritic cells-CIK was the strongest, up to (77.88± 1.57)% (P < 0.01). It also showed that dendritic cells-CIK has a stronger anti-tumor activity, and the toxicity of dendritic cells-CIK against leukemia cells was significantly higher than simple application of CIK or dendritic cells.

CLC Number: