Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9185-.doi: 10.3969/j.issn.1673-8225.2010.49.015

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Repairing articular cartilage defects with cartilage-derived morphogenetic protein transfected autologous bone marrow mesenchymal stem cells

Mu Chang-zheng, Ma Yun-sheng, Wang Zheng   

  1. Department of Histology and Embryology, Liaoning Medical University, Jinzhou  121000, Liaoning Province, China
  • Online:2010-12-03 Published:2010-12-03
  • About author:Mu Chang-zheng★, Master, Professor, Master’s supervisor, Department of Histology and Embryology, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China muchangzheng@sina.com
  • Supported by:

    the Innovation Team Program Foundation of Department of Education of Liaoning Province, No. 2006T062*; the Natural Science Foundation of Liaoning Province, No. 20072201*

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Abstract:

BACKGROUND: It can repairing cartilage defect somehow with scarffolds or stem cells, but we found that the effect of repair were not satisfied in large defects. The gene transfected stem cells can continuously release grow factors, and this may be presented a progress in cartilage defect.
OBJECTIVE: To verify whether cartilage-derived morphogenetic protein 1 (CDMP1)-transfected autologous bone marrow mesenchymal stem cells (BMSCs) enhance cartilage repair in rabbit in vivo.
METHODS: BMSCs were isolated from bone marrow of rabbits, and transfected with the CDMP1 gene by the lipofection method. The autologous cells were then implanted into full-thickness articular cartilage defects in the knee joints of rabbit as experimental group; BMSCs were transplanted as control BMSCs group and no cells transplanted as blank control group. The repair was analyzed by histological detection and histological score following surgery.
RESULTS AND CONCLUSION: During in vivo repair of articular cartilage defects, cartilage regeneration was enhanced by the implantation of CDMP1 transfected autologous BMSCs. The defects were filled by hyaline cartilage and the deeper zone showed remodeling to subchondral bone overtime. There pair and reconstitution of zones of hyaline articular cartilage was superior to simple BMSCS implantation. The histological score of the CDMP1 transfected BMSCs group was significantly better than those of the BMSCs control group and the blank control group. These indicate that CDMP1 transfected BMSCs enhanced repair and remodeling of articular cartilage.

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