Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9181-.doi: 10.3969/j.issn.1673-8225.2010.49.014

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Changes in motor function and evoked potentials of rats with acute spinal cord injury following olfactory ensheathing cells transplantation combined with Methyprednisolone

Wang Bin1, He Xi-jing2, Li Qiang3, Wang Wei-xiong1, Shen Bo1   

  1. 1 Department of Trauma Emergency Surgery, the Second Affiliated Hospital of Guangzhou Medical College, Guangzhou   510260, Guangdong Province, China; 2 Second Department of Orthopaedics, the Second Affiliated Hospital of Xi’an Jiaotong University Medical College, Xi’an   710004, Shaanxi Province, China; 3 Department of Orthopaedics, East Branch of Qingdao Municipal Hospital, Qingdao   266011, Shandong Province, China
  • Online:2010-12-03 Published:2010-12-03
  • About author:Wang Bin☆, Doctor, Associate chief physician, Department of Trauma Emergency Surgery, the Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, Guangdong Province, China wbhngz@126.com
  • Supported by:

    Startup Foundation for Doctors in Guangzhou Medical College*

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Abstract:

BACKGROUND: Olfactory ensheathing cells (OECs) transplantation and Methyprednisolone (MP) are identified as two very promising methods in treating spinal cord injury (SCI). However, there are few reports about OECs combined with MP in treating SCI, and the treatment results are different.
OBJECTIVE: To investigate the behavioral score and electrophysiological effects of OECs transplantation and MP on the repair of acute spinal cord injury and whether there is a synergistic effect between OECs transplantation and MP.
METHODS: Acute spinal cord injury model (T10) was established by NYU impactor. All rats were randomly divided into 6 groups: control group, SCI group, DF12 group, OECs transplantation group, MP group, and OECs + MP group. Rats were injected with OECs, MP, OECs + MP, DF12 serum-free medium and saline, respectively after operation. At 8 weeks following operation, motion evoked potential (MEP) and somatosensory evoked potential (SEP) tests were performed. Changes in motor function before and after operation were estimated by Basso-Beattie-Bresnahan (BBB) score.
RESULTS AND CONCLUSION: Compared with the SCI and DF12 groups, BBB score was increased, the latency period in SEP and MEP was decreased and wave amplitude was increased in the OECs, MP, and OECs + MP groups at 8 weeks following operation. Significant difference was detected (P < 0.05). The latency period in SEP and MEP was decreased and wave amplitude was increased in the OECs + MP group compared with the OECs group and MP group, and significant difference was detected (P < 0.05). OECs transplantation and MP alone can significantly promote recovery of motor function of acute spinal cord injury rats. OECs transplantation combined with MP can more significantly promote recovery of the motor function of acute spinal cord injury rats.

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