Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9172-.doi: 10.3969/j.issn.1673-8225.2010.49.012

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Autologous bone marrow mesenchymal stem cell transplantation via lumbar puncture in the treatment of cerebral infarction sequelae in 6 cases

Jiao Hong-liang 1,2 , Guan Fang-xia 3, Yang Bo1, Li Jian-bin2, Zhan San-hua4, Du Ying5, Hu Xiang6, Zhao Lin-na 2, Han Xiao-gai2   

  1. 1 Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 2 Henan Province Red Cross Blood Center, Zhengzhou  450014, Henan Province, China; 3 Department of Biomedical Engineering, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 4 Department of General Internal Medicine, Henan Province Armed Police Forces Hospital, Zhengzhou  450052, Henan Province, China; 5 Department of Microorganism and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 6 Beike Bio-Technology Co., Ltd., Shenzhen  518000, Guangdong Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Yang Bo, Doctor, Professor, Doctoral supervisor, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Jiao Hong-liang, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Henan Province Red Cross Blood Center, Zhengzhou 450014, Henan Province, China jhl787878@126.com
  • Supported by:

    the Key Subject Construction Project of 211 Engineering of Zhengzhou University*; the Medical Science Technology Innovation Talent Engineering Program of Henan Province, No. 2005018*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells have multi-directional differentiation potential, and are easily, rapidly expanded, low immunogenicity, avoid immunological rejection in the transplant process, are ideal seed cells in the treatment of neurological diseases such as cerebral infarction.
OBJECTIVE: To explore the safety, feasibility and efficacy of autologous bone marrow mesenchymal stem cell after culture transplantation via lumbar puncture in treatment of cerebral infarction.
METHODS: A total of 6 cases of sequelae of cerebral infarction received autologous bone marrow mesenchymal stem cell transplantation. Autologous bone marrow mononuclear cells were collected by bone puncture. Bone marrow mesenchymal stem cells in vitro cultured 4 days after transplantation by lumbar puncture in the subarachnoid space. The efficacy and complications were observed after transplantation.
RESULTS AND CONCLUSION: Numbers of BMSCs were significantly increased after culture. There were significant differences in NIHSS scores and muscle strength of patients before and after operation (P < 0.05) and the overall improvement of symptoms were improved significantly. After transplantation, there were no serious adverse events. The results suggest that autologous bone marrow mesenchymal stem cell transplantation can significantly improve symptoms in patients with cerebral infarction sequelae; treatment is safe, effective and low side effects.

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