Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9159-.doi: 10.3969/j.issn.1673-8225.2010.49.009

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Effect of bone marrow mesenchymal stem cell transplantation on alveolar fluid clearance in acute lung injury rats

Zhang Jun-zhi, Wang Ping, Yao Shang-long, Liu Tang-hua, Gui Ping   

  1. Department of Anesthesiology, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan  430022, Hubei Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Yao Shang-long, Professor, Doctoral supervisor, Department of Anesthesiology, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China yaoshanglong1277@126.com
  • About author:Zhang Jun-zhi☆, Studying for doctorate, Department of Anesthesiology, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China zjz_1980@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30930089*

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Abstract:

BACKGROUND: The increase of alveolar fluid is an important pathophysiological change of acute lung injury. Repairing the damaged alveolar-capillary membrane barrier is an effective way of alleviating pulmonary edema.
OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on alveolar fluid clearance in endotoxin-induced acute lung injury rats.
METHODS: Rat BMSCs were isolated and cultured using the modified Peister method. At the third passage, the BMSCs were made into single cell suspension. The rats were randomly divided into three groups. In the acute lung injury and cell transplantation groups, endotoxin was injected into the abdomen to establish models of acute lung injuny. One hour following model establishment, the intervention group, by caudal vein injection, was injected with BMSC suspension of 0.5 mL (cell count 1 × 106), and the control group and damage group were given the equal volume of normal saline. For each group, the rate of alveolar fluid clearance was measured, the lung water content level was assayed, and the pathological changes of lung tissue were observed after tail vein injection at 6, 24 and 48 hours respectively.
RESULTS AND CONCLUSION: The lung water content level assay showed that the wet to dry ratio for lung injury group was significantly higher (P < 0.05) at 24 hours, compared with the normal control group and cell transplantation group. Compared with the lung injury group, the wet to dry ratio of the cell transplantation group was significantly lower (P < 0.05) at various time points. For the cell transplantation group, the rate of alveolar fluid clearance was significantly higher at various time points, compared with the lung injury group (P < 0.05). By hematoxylin-eosin staining microscopy, the lung injury group showed capillary congestion and alveolar infiltration of inflammatory cells at 6 hours, which aggravated at 24 hours. The cell transplantation group showed that at 6 hours the alveolar space infiltration of inflammatory cells was better, compared with the lung injury group, which continued to get better at 24 hours and become basically close to the normal control group at 48 hours. This indicated that BMSC transplantation may be able to promote the alveolar fluid clearance in acute lung injury by repairing damaged alveolar-capillary membrane barrier.

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