Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9155-.doi: 10.3969/j.issn.1673-8225.2010.49.008

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Pravastatin effects on steroid-induced differentiation of bone marrow stromal cells

Wen Guo-hong1, Xiao Zeng-ming2   

  1. 1 Third Department of Orthopaedics, Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan  442000, Hubei Province, China; 2 Department of Spine Orthopaedics, First Affiliated Hospital of Guangxi Medical University, Nanning  530071, Guangxi Zhuang Autonomous Region, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Xiao Zeng-ming, Department of Spine Orthopaedics, First Affiliated Hospital of Guangxi Medical University, Nanning 530071, Guangxi Zhuang Autonomous Region, China wenguoh@yahoo.com
  • About author:Wen Guo-hong★, Master, Chief physician, Third Department of Orthopaedics, Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, Hubei Province, China wenguoh@yahoo.com

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Abstract:

BACKGROUND: Pravastatin as a hypolipidemic drug is widely used in clinic. With the deep research of this kind of drugs, it has effects on bone formation. Whether pravastatin can inhibit the differentiation of hormone-induced bone marrow stromal cells into adipocytes remains poorly understood.
OBJECTIVE: On the base of steroid-induced adipogenesis in bone marrow stromal cells, to observe the influence of pravastatin and comment on the mechanism of prevention and treatment of steroid-induced necrosis of femoral head.
METHODS: Bone marrow of bilateral femur of young rabbits was obtained, and bone marrow stromal cells were isolated by cell adherence. The osteoprogenitor cells were treated with dexamethasone which acted as adipocyte differentiation inducer and various mass concentrations of pravastatin. The cells in culture were stained on day 21 following culture. The number of adipocytes was observed under a light microscope, and counted on a pathological image analyser. Cultured cells received immunohistochemical staining on day 12. Activities of alkaline phosphatase of the cells were measured.
RESULTS AND CONCLUSION: On day 21 following culture, the number of adipocytes in each group was reduced with the increased dose of pravastatin, in a dose dependent fashion. In addition, on day 21 following treatment of dexamethasone and different mass concentrations of pravastatin, immunohistochemical method confirmed that the activities of alkaline phosphatase were significantly increased. Results suggest that dexamethasone can induce the differentiation of bone marrow stromal cells into adipocytes, and reduce osteoblast differentiation. Pravastatin can suppress the differentiation of bone marrow stromal cells into adipocytes, and contribute to osteoblast differentiation.

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