Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9151-.doi: 10.3969/j.issn.1673-8225.2010.49.007

Previous Articles     Next Articles

Proliferation of bone marrow mesenchymal stem cells under different serum culture conditions

Wu Gang, He Hui, Cao Yue-cheng, Chen Xu-chun, Yang Lei, Fu Da-zhi   

  1. General Surgery and Organ Transplantation Unit, First affiliated Hospital, China Medical University, Shenyang  110001, Liaoning Province, China
  • Online:2010-12-03 Published:2010-12-03
  • About author:Wu Gang☆, Doctor, General Surgery and Organ Transplantation Unit, First affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China WGZWL@hotmail.com
  • Supported by:

    he Key Laboratory Program of Department of Education of Liaoning Province, No. 20060903*

PDF

666

Abstract:

BACKGROUND: There are many studies concerning bone marrow mesenchymal stem cells for culturing methods, and fetal bovine serum (FBS)/ fetal calf serum (FCS) are used widely. However, there is few study about cord blood serum (CBS) culture method on bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To explore the effects of different serum culture method on the proliferation of BMSCs.
METHODS: Ficoll density gradient centrifugation method was used to isolate and purify BMSCs that were incubated in L-DMEM containing CBS and FBS and serum-free media. Morphology and proliferation of BMSCs were observed. CD34, CD44, CD45 and CD105 were identified using flow cytometry.
RESULTS AND CONCLUSION: CBS group cells appeared smaller, more spindle-shaped with fewer cytoplasmic processes than the cells cultured with FBS. CBS and FBS could promote cell growth and proliferation, but BMSCs in CBS were active, but no proliferation was determined in serum-free media. Flow cytometry showed that BMSCs were negative for CD34, CD45, but positive for CD105 and CD44. Surface marker on BMSCs did not significantly change following CBS amplification.

CLC Number: