Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9147-.doi: 10.3969/j.issn.1673-8225.2010.49.006

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Comparison of biological characteristics of rat bone marrow stromal stem cells at different ages

Zhao Fu-sheng 1, Wu Geng 2, Wu Yang3, Jin Xiu-dong4, Zhang Ji-fei1   

  1. 1 Department of Histology and Embryology, 2 Clinical Technical Skill Training Center, 4Department of Physiology,Mudanjiang Medical University, Mudanjiang  157011, Heilongjiang Province, China; 3Electrocardiographic Room, Hongqi Hospital, Mudanjiang Medical University, Mudanjiang  157011, Heilongjiang Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Zhang Ji-fei, Professor, Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China zhangjifei777@163.com
  • About author:Zhao Fu-sheng★, Master, Lecturer, Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China zhaofusheng910@163.com
  • Supported by:

    the Science and Technology Research Program of Department of Education of Heilongjiang Province, No. 11551522*

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Abstract:

BACKGROUND: Bone marrow stromal stem cells (BMSCs) have multiple differentiation abilities. BMSCs are easily obtaining, have strong proliferative ability and can do autologous transplantation. Therefore, it is an ideal seed cells in tissue engineering. Few studies have addressed effects of age on biological characteristics of BMSCs.
OBJECTIVE: To compare differences in biological characteristics of BMSCs from rats at different ages, and to provide experimental evidence for clinical cell transplantation.
METHODS: BMSCs were obtained from Sprague-Dawley rats. SD rats were divided into three groups according to the source of cells, namely childhood group, adult group, gerontism group. The femur at both ends was cut under sterile conditions. Bone marrow cavity was washed using DMEM medium containing 10% fetal bovine serum. Bone marrow fluid was collected. Single cell suspension was made, and placed in culture flask after filtration through 200 meshes. The second passage of cells was obtained. CD44, CD29, CD90 protein expression was determined using immunofluorescence staining. Morphology and adhesion rate of BMSCs were observed. The cell growth curve was drawn by cells counting. Activity of BMSCs was detected using MTT assay.
RESULTS AND CONCLUSION: The cells were positive for the CD44, CD29 and CD90 immunostaining. The adhesion rate of childhood group was faster than adult and gerontism groups as well as the proliferation rate (P < 0.05). MTT assay showed that the viability of childhood group was significantly stronger than the other two groups (P < 0.05). Results indicated that BMSCs from the young rats are the ideal tissue engineering cells, for the characters of dynamic and fast amplification in a short time.

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