Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9131-.doi: 10.3969/j.issn.1673-8225.2010.49.003

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Gene expression during induced differentiation of bone marrow mesenchymal stem cells into osteoblasts

Hu Yang, Ma Ying, He Hui-yu, Sheng Lei, Abulizi•Abudula, Aerziguli   

  1. Department of Prosthodontics, First Affiliated Hospital, Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: He Hui-yu, Doctor, Associate professor, Associate chief physician, Department of Prosthodontics, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China joe98344@sina.cn
  • Supported by:

    Key Scientific Program of Xinjiang Uygur Autonomous Region, No. 200533118*; the Scientific Research Key Program of Scientific Research Project of Higher Learning School of Xinjiang Uygur Autonomous Region, No.xjEDU2009I22*

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Abstract:

BACKGROUND: At present, the gene expression mode of bone marrow mesenchymal stem cells (BMSCs) differentiation into osteoblasts remains unclear.
OBJECTIVE: To observe gene expression of alkaline phosphatase, osteopontin, type Ⅰcollagen, basic fibroblast growth factor and osteocalcin during BMSCs differentiation into osteoblasts, and to verify whether differentiation of BMSCs into osteoblasts is mature.
METHODS: The BMSCs were aspirated from the femoral bone of 2-month old New Zealand rabbits and cultured by all bone marrow adherence method, and induced to differentiate into osteoblasts in the medium supplemented with DMEM/F12, dexamethason 1×10-8 mmol/L, β-glycerophosphate sodium 0.01 mol/L and vitamin C 0.05 g/L. The gene expression of alkaline phosphatase, osteopontin, type Ⅰcollagen, basic fibroblast growth factor and osteocalcin in the first and second passages of BMSCs were examined by reverse transcription-polymerase chain reaction. BMSCs surface antigen CD44 was identified.
RESULTS AND CONCLUSION: Following induced culture in mineralized media, the first and second passages of BMSCs expressed alkaline phosphatase, osteopontin, type Ⅰcollagen, basic fibroblast growth factor and osteocalcin gene in some phases. Positive rate of CD44 in the first passage of BMSCs was 44.4%. These indicate that rabbit BMSCs gradually differentiated into osteoblasts following in vitro mineralization. The first and second passages of BMSCs expressed alkaline phosphatase, osteopontin, type Ⅰcollagen, basic fibroblast growth factor and osteocalcin in some phases. These cells have had the characteristics of osteoblasts and provided experimental evidences for revealing the mechanisms of gene expression of BMSCs differentiation into osteoblasts.

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