Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (49): 9121-.doi: 10.3969/j.issn.1673-8225.2010.49.001

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Effects of neotype bone morphogenetic protein-7 peptide on biological behavior of bone marrow stromal stem cells

Feng Bo 1,2, Hu Dong-xu 1,3, Zhang Yang-de 1,3, Wu Yu-chi2   

  1. 1 Institute of Biomedical Engineering of Central South University,  Changsha  410008, Hunan Province, China; 2 Department of Orthopaedics, Baogang Hospital, Third Affiliated Hospital of Inner Mongolia Medical College, Baotou  014010, Inner Mongolia Autonomous Region, China; 3 Research Center of Liver, Gall and Intestine Surgery, Ministry of Health, Changsha  410008, Hunan Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Zhang Yang-de, Doctoral supervisor, Chief physician, Professor, Institute of Biomedical Engineering of Central South University, Changsha 410008, Hunan Province, China; Research Center of Liver, Gall and Intestine Surgery, Ministry of Health, Changsha 410008, Hunan Province, China
  • About author:Feng Bo☆, Studying for doctorate, Chief physician, Institute of Biomedical Engineering of Central South University, Changsha 410008, Hunan Province, China; Department of Orthopaedics, Baogang Hospital, Third Affiliated Hospital of Inner Mongolia Medical College, Baotou 014010, Inner Mongolia Autonomous Region, China bb0472111@yahoo.com.cn

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Abstract:

BACKGROUND: The study of bone morphogenetic protein-7 (BMP-7) mainly focused on its application to tissue engineering. However, BMP-7 has strong bone induction activity and affects growth, development and physiological function of animals’ reproductive system. How to fully exert its bone induction activity and to avoid other biological effects is a difficulty in bone tissue engineering field.
OBJECTIVE: To explore the effect of BMP-7 peptide on proliferation, osteogenic differentiation and mineralization of bone marrow stromal stem cells (BMSCs).
METHODS: BMSCs were harvested from rats and identified with flow cytometry. The 3rd generation of BMSCs was divided into experimental and control groups. The medium of experimental group was supplemented with 100 mg/L BMP-7 peptide. The medium of control group was not supplemented with BMP-7 peptide. After 2, 4, 6, 8, and 10 days, the proliferation of BMSCs was analyzed by MTT assay. After 7 and 14 days, the calcium contents and alkaline phosphatase activities were measured to assess the differentiation of BMSCs towards osteoblasts. The mineralization of BMSCs was determined by calcein staining.
RESULTS AND CONCLUSION: BMSCs grew well in primary culture and the purity of BMSCs was 91.5%. There was no significant difference in proliferation between experimental and control groups (P > 0.05). On days 7 and 14, calcium contents and alkaline phosphatase activities in the experimental group were significantly higher than the control group (P < 0.05). The mineralization level of BMSCs in the experimental group was significant higher than in the control group. Results suggest that BMP-7 peptide has no influence on the proliferation of BMSCs, but it can promote osteogenic differentiation and mineralization of BMSCs significantly.

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