Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (45): 8446-8449.doi: 10.3969/j.issn.1673-8225.2010.45.020

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Intervention role of ground beetle on the expression of Type Ⅰ collagen mRNA in steroid-induced bone marrow mesenchymal stem cells

Qi Zhen-xi1, Li Shu-qiang2, Yu Tao2   

  1. 1 Institute of Osteopathy, Fujian Academy of Integrative Medicine, Fuzhou  350108, Fujian Province, China; 2 Department of Bone Injury, Fujian University of Traditional Chinese Medicine, Fuzhou  350108, Fujian Province, China
  • Online:2010-11-05 Published:2010-11-05
  • About author:Qi Zhen-xi☆, Doctor, Professor, Institute of Osteopathy, Fujian Academy of Integrative Medicine, Fuzhou 350108, Fujian Province, China zxqi@fjtcm.edu.cn
  • Supported by:

    the Science and Technology Project of Fujian Province, No. 2007F5060*

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Abstract:

BACKGROUND: The early pathological changes of steroid-induced avascular necrosis of femoral head may be related to the steroid-induced bone marrow mesenchymal stem cells (BMSCs) adipogenic differentiation.
OBJECTIVE: To investigate the promoting effects of ground beetle drug-containing serum on osteogenic differentiation of BMSCs.
METHODS: The 3rd generation of BMSCs was randomly divided into blank group, control group and Chinese medicine with high, moderate and low dose groups. The low-, moderate- and low-dose Chinese medicine groups were treated with various doses of medium (1 L medium contained 100 g drug-containing serum), with an additional dexamethasone till final concentration was
10-6 mol/L. The control group was subjected to medium containing saline serum, with an additional dexamethasone till the final concentration was 10-6 mol/L. The medium containing saline serum was added in the blank group, without dexamethasone. At 6 days following intervention, ossify markers Collagen I mRNA and alkaline phosphatase (ALP) expression in cells was determined.
RESULTS AND CONCLUSION: Following 6 days of intervention, the intracellular ALP content and mRNA expression of collagen type I was significantly lower in the control group than the blank group, while was greater in the Chinese group than in the control group, and the differences were significant (P < 0.01). These suggest that mechanism of ground beetle to prevent and to cure the avascular necrosis of the femoral head is not only to improve the microcirculation, but also associates with the decrease in steroid-induced osteogenic differentiation of BMSCs.

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