Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7551-7554.doi: 10.3969/j.issn.1673-8225.2010.40.032

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Characteristics and mechanisms of mesenchymal stem cell culture and induced differentiation　

Zhao Lin, Shen Yan-qing, Rong Chun, Zhou Yan-bing, Xia Chang-suo   

  1. 1 Operation Room, 2 Department of General Surgery, 3 Department of Orthopaedics, Hospital Affiliated to Medical College, Qingdao University, Qingdao  266003, Shandong Province, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Zhou Yan-bing, Master, Professor, Department of General Srugery, Hospital Affiliated to Medical College, Qingdao University, Qingdao 266003, Shandong Province, China 266003 xcs009@163.com Xia Chang-suo, Doctor, Associate professor, Department of Orthopaedics, Hospital Affiliated to Medical College, Qingdao University, Qingdao 266003, Shandong Province, China Xcs009@163.con
  • About author:Zhao Lin★, Studying for master’s degree, Associate chief nurse, Operation Room, Hospital Affiliated to Medical College, Qingdao University, Qingdao 266003, Shandong Province, China yzhsyq666@163.com
  • Supported by:

    the Doctor Scientific Research Fund of Hospital Affiliated to Medical College of Qingdao University, No. 2006-21*　

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Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) are a class of adult stem cells with multi-directional differentiation potential.
OBJECTIVE: To review MSCs separation, identification, characterization and application prospects.
METHODS: The Medline database was searched for articles published from January 1996 to March 2010. The key words were “mesenchymal stem cells”. The China National Knowledge Infrastructure database was retrieved for articles published from 1979 to February 2010. The key words were “mesenchymal stem cells, culture, differentiation”.
RESULTS AND CONCLUSION: A total of 400 articles concerning MSCs isolation, culture, identification and differentiation were collected, including 86 Chinese articles and 314 English articles. Published earlier, repeated articles and those with similar studies were excluded, and 36 articles in accordance with inclusion criteria were included. We found that there are many methods concerning MSCs isolation, culture and identification, but there is no uniform standard, the mechanism of inducing cell differentiation remains unclear, and there are many problems in application.　

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