Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7529-7532.doi: 10.3969/j.issn.1673-8225.2010.40.026

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Mononuclear cells and platelets isolated from human bone marrow using hydroxyethyl starch by one-step way versus two-step way

Ma Yan1, Chen Shuang 2, Bi Xiao-juan 1, Jiang Ming 1, 2   

  1. 1 Stem Cell Laboratory, Medical Research Center, 2 Institute of Hematology, First Affiliated Hospital, Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Jiang Ming, Professor, Master’s supervisor, Chief physician, Stem Cell Laboratory, Medical Research Center, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Institute of Hematology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China jiangmingyy@yahoo.com.cn
  • About author:Ma Yan, Associate chief technician, Stem Cell Laboratory, Medical Research Center, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China mayan1121@126.com

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Abstract:

BACKGROUND: It can improve the concentrated efficiency of mononuclear cell to use hydroxyethyl starch with lymphocyte separating medium. However, there is no report addressing the residual volume of platelets.
OBJECTIVE: To detect the efficiency of isolating mononuclear cells and residual volume of platelets using hydroxyethyl starch by one step or two-step way.
METHODS: Bone marrow was obtained from the samples of patients treated with adult autologous bone marrow karyocyte transplantation performed at the First Affiliated Hospital, Xinjiang Medical University, China. Bone marrow tissues acquired from patients were sedimentated by hydroxyethyl starch solution only, or followed by further centrifugation with lymphocyte separating medium. Karyocytes, mononuclear cells and platelet number by these two methods were observed.
RESULTS AND CONCLUSION: The average percentage of mononuclear cells in two-step group (89%) was significantly higher than one-step group (45%) (P < 0.05). Moreover, the number of platelets was lower in two-step group (median: 73.00) than one-step group (median: 367.50) (P < 0.05). Results have suggested that the isolation efficiency of using hydroxyethyl starch solution by two-step way from human bone marrow was higher than one-step way.

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